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From 2013.igem.org

　　

1>LB solid recipe(1L):
　yeast extract 5g
　agarose(100ml 1.5g)
　sodium chloride 10g
　peptone 10g.
　and the liquid recipe, based on the materials given above,

2>Calculate the reagents we need, preparation for 400ml and 900ml LB, and then weigh the relevant reagents and dissolve them.

3>Autoclave the culture to fully dissolve the reagents.