SUSTC/15 August 2013

From 2013.igem.org

2013-08-15

We had to admit that we encountered a confused situation when we implemented our first self attempt, and we were hopeful that the bacteria community appearing on the plate yesterday would give fluorescence today, but eventually it turned out to be a bad result. What was meaningful was that we operated an advanced machine-flow cytometry, which was very useful in detecting the fluorescence and the growth conditions and the types of the bacteria.

We fetched one tube of bacteria solution cultured yesterday and diluted 20 times, then we added xylose until the xylose concentration reached 0.003, 0.03, 0.3, 3, 0.01, 0.1, 1M. After they were cultured for 3 hours, we took them to the Shenzhen advanced technology research center to use the flow cytometry.

Defeat analysis:
1. Low xylose concentration;
2.the xylose functions for a long time, but the bacteria were cultured for a short time;
3.DNA molecular hadn’t been imported into the bacteria;4.something was wrong with the DNA. <p>Solutions: <ui>

  • Dilute the 1 bacteria solution 20 times and prepare 4% and 8% xylose solution;</li>
  • Dilute the 2 bacteria solution 20 times and prepare 4% and 5% xylose solution;</li>
      Then culture them.