SUSTC/18 July 2013

From 2013.igem.org

2013—07-18

The first day of our experiments, in fact, our training. All of us were a little excited for the sake of the start of our experimental process.

Washing the glass media bottles.(1L bottle contains 900ml at most, 500ml bottle contains 400ml at most.) Combination of the solution: LB solid recipe(1L)—yeast extract 5g, agarose(100ml 1.5g), sodium chloride 10g, peptone 10g.

We prepared two bottles of LB solid solution, the volume of which are both 900ml. Then we prepared two bottles of LB liquid solution, the volume of which are both 400 ml(without adding agarous into it).

Autoclaving: getting the LB solid and liquid solution, tips, and EP tubes sterilized.

Attention:

  • 1. Before deserting the EP tubes which have been used, they must be coped with the NaClO solution for half an hour.
  • 2. When sterilized, the cap of the glass media bottle could not be tightly twisted.