SUSTC/30 August 2013

From 2013.igem.org

2013-08-30

It seemed that god was fond of playing jokes on us and good things should be pairs. After continuous defeats, we got continuous success now. The experiments done yesterday was a big success as well. The red fluorescence given by the 5m M nitrate bacteria was apparent even on the daylight. The outcome turned to be excellent, and other plates had good conditions as well.

Then we prepared 40% glycerol, and preserved all types of the bacteria we got. Since the preliminary experiment on the nitrate was a huge success, we decided to do the concentration gradient experiment. We cultured the bacteria in different nitrate concentration-0m M, 5m M, 10 m M, 15m M, 20m M.