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Small Phage
From 2013.igem.org
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Overview Winter Summer Fall |
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Contents |
March
April
May
5/1/13
- Commencement of spring!!!
- Discussed goals and outlined plans for spring term
5/2/13
- Designed primers for amplifying and sequencing phage capsid protein
- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly
5/3/13
- Performed agar test, focusing primarily on ×8
- Processed phage amplification
5/4/13
- Performed dilution series using stock 5.3 (-1 through -11)
- Started two 5mL overnights of BL21
5/5/13
- Spot test using stock 5.3 and its dilution series (from 5.4)
- Started liquid culture for purification team (at around noon)
- 1mL of BL21 overnight + 4mL of LB + 100μL -2 phage (received stock)
- Started 5.5 amplification from a plaque test
5/6/13
- Continued 5.3 T7 phage amplification/purification
- Performed liquid culture phage concentration test
5/7/13
- Started two 5mL of E coli BL21 overnight
- Designed procedure for applying mutagen and selecting for T7
5/8/13
- Went over procedure for applying mutagen and PCR with Dr. Grose
- Performed spot tests under 5.6 T7+ Liquid Culture Phage Concentration Test
- Started two 5mL BL21 overnights
- Learned how to create LB plates
5/9/13
- Practiced with ×6 and ×8 top agar
- Started 5.9 T7+ Liquid Culture Phage Concentration Test #2
- Discussed plans and schedule for next week.
5/20/13
LP, XL
- We performed T7 Mutagen Concentration Test
- We performed T7 Minor Capsid Protein PCR
5/21/13
XL
- started two 5mL E coli BL21 overnight at around 7:00pm
5/22/13
- Continued with T7 Mutagen Concentration Test
- Continued with T7 Minor Capsid Protein PCR by running an agarose gel to confirm that we got the desired PCR product.
June
July
August
September
