Team:BYU Provo/Notebook/Phage Purification/Springexp/Period4/Dailylog

From 2013.igem.org


Phage Purification May - June Notebook: June 10 - June 23 Daily Log



Phage Purification
March-April
May-June
July-August
September-October



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6/10/13

- Prepared RNase A for future use.

6.10 RNase A Preparation

- Prepared T4 and T7 cultures.

6.10 Phage Preparation


6/12/13

- Began first step of purification process using phage from 6.10 Phage Preparation.

6.12 PEG Purification


6/14/13

- Worked on updating the website.


6/17/13

- Set up CsCl gradient 6.17 CsCl Gradient

- Prepared T4 and T7 cultures.

6.17 Phage Preparation



6/19/13

- Began first step of purification process using phage from 6.17 Phage Preparation.

6.19 PEG Purification

- Ran spot tests on collected band from 6.17 CsCl Gradient.

6.19 Spot Test



6/20/13

- Finished 6.19 PEG Purification by centrifuging solution at 10,000 g 15 minutes at 4 C, removing supernatant, and suspending the pellet in phage suspension buffer.


6/21/13

We centrifuged the pellet at 10,000 g for 15 min at 4 C after suspending it to clean up the walls of the tube.


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