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• iGEM 2013

Phage Purification July - August Notebook: Experiments

Phage Purification March-April May-June July-August September-October

7.10 T7 Phage Pictures I) Purpose To see if we have mutated, pure phage from the CsCl gradient and mutagenisis II) Expected Outcome Pictures of pure, intact phage, potentially mutated phage. III) Reagants Used Prepared Phage, T7 IV) Actual Procedure Gave prepared phage to TEM operator. We had one sample of T7 phage from CsCl purifictaion. V) Results Pictures of T7 Phage We noticed large amounts of crystal structure and a low concentration of phage. In the future, we will use larger concentrations of phages and run another round of dialysis.