Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/4.2W311050mLLiquidCulture

From 2013.igem.org


Phage Purification March - April Notebook: Experiments



Phage Purification
March-April
May-June
July-August
September-October

4.2 W3110 50 mL Liquid Culture


I) Purpose

Grow bacteria for use in experiments.

II) Expected Outcome

Viable liquid culture of E. coli, W3110

III) Reagants Used

W3110 plated colonies
LB


IV) Actual Procedure

We put a colony from our W3110 plate of E.coli into 50mL LB broth and left in shaker at 37 overnight.


V) Results

Growth of W3110 liquid cultures of bacteria for use in phage amplification.
The control tube showed no cantamination.