Team:Calgary/Notebook/Protocols/RehydrationOfRegistryDNA

From 2013.igem.org

Rehydration of Registry DNA

Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.

Reagents and Reagents

  • Biobrick parts
  • ddH2O (deionized water)

Protocol

  1. Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want
  2. Add 10 μL of ddH2O (deionized water)
  3. Let the water incubate at room temperature for 10 minutes
  4. Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies