Team:DTU-Denmark/Notebook/19 September 2013

From 2013.igem.org

19 September 2013

Navigate to the Previous Entry

Contents

Lab 115


Main purpose


The purpose of this experiment is to measure nitric and nitrous oxide from the Nir transformant anaerobically while we spike it with nitrite.

The Experiment 2 describes further our investigation.

Who was in the lab


Kasia, Ariadni

Procedure


This experiment is based on the Protocol of Experiment 2 for the Nir transformant, with some changes.

The experiment started with a temperature of around 37 o C, initial OD of 0.1036 and initial volume of 200 ml. After resuspension the OD was really low around 0.0329.

The experiment starts, taking one sample and after 7 minutes, a second spike and then we spike with 2ml of nitrite and we take two samples. Afterwards we also spike with 2 ml of nitrite again and finally take one more sample.

Results


Colorimetric results

Ranges

  • Measuring range 5-150 mg/L NH4-N
  • Measuring range 0.02-1 mg/L NO2-N


salt (mg/L) sample 1 sample 2 sample after spike sample 4 sample 5
ammonium <2 <2 <2 <2 <2
nitrite <0.02 <0.02 0.74 1.24 1.34


Nir Transformed E. coli

Dtu-nir.png

Conclusion


The NO sensor was drifting and no proper results were obtained.


Navigate to the Previous Entry