Team:DTU-Denmark/Notebook/1 July 2013

From 2013.igem.org

1 July 2013

Contents

208 lab


Main purpose today


Make M9 Medium for the biolector toxicity experiment

Who was in the lab


Helen, Gosia, Ariadni

Procedure


According to open wetware protocol for Biolector Experiment

1L of Media:

  • 10 mL Casamino Acid ( ready solution from Andreas)
  • 2 mM MgSO4 (from 2M liquid stock)
  • 0.1 mM CaCl2 (liquid solution with 15% gly)
  • 200 mL M9 salts
  • Fill to 1 L with sterilized water

1 L stock of M9 salts:

  • 64 g Na2HPO4*7H2O = 31.2 g Na2HPO4
  • 15 g KH2PO4
  • 25 g NaCl
  • 5 g NH4Cl

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