Team:Exeter/Parts

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Exeter iGEM 2013 · Paint by Coli

Parts

This page will document the parts that Exeter iGEM 2013 submit to the biobrick registry.

Our aim is to submit a composite biobrick for E. coli which will allow control three genes of your choice using red, green and blue (RGB) light. We have successful sent in the composite part for the green light module ([1]). We also aim to create constituent bio-bricks for red/blue/green light input matching cyan/yellow/magenta pigments respectively. We have managed to submit a brick for the red light module (includes the cyan pigment, see [2]). We will also submit a constituent bio-brick for red light input with cut sites to allow input of your favourite gene ([3]). See our project section for details about our bio-bricks.

We plan to extensively characterise our system and provide a mathematical model of our system for quantative control of outputs. See our modelling section.

Table including all of the parts we are hoping to utilise

Part Code and link to Registry page Name of Part
BBa_K608002 Strong promoter and strong RBS
BBa_I712074 T7 promoter
BBa_B0015 Terminator
BBa_Q04510 lambda cI inverter system
BBa_K322124 Cph8 red light sensor with RBS, version one
BBa_K592018 Cph8 red light sensor with RBS, version two
BBa_K098011 OmpR, the protein which communicates between the red light sensor and the cyan pigment gene
BBa_R0082 OmpR specific promoter, only allows transcription when OmpR-P is present
BBa_K322122 Cyan pigment, version one (composed of HO1 and pcyA, makes phycocyanobillin)
BBa_K909000 Cyan pigment, version one, includes stronger RBS than BBa_K322122
BBa_K592011 Cyan pigment, version two
BBa_K864404 Cyan pigment, version two, includes a promoter and RBS
BBa_K592022 Cyan pigment, version two, includes a alternative promoter and RBS
BBa_K592004 YF1 blue light sensor
BBa_K592005 FixJ, the protein which communicates between the blue light sensor and the yellow pigment gene
BBa_K592006 FixJ specific promoter, only allows transcription when FixJ-P is present
BBa_K592010 Yellow pigment, version one
BBa_S05058 Yellow pigment, version one, with added RBS
BBa_K592001 CcaS green light sensor
BBa_K592002 CcaR, the protein which communicates between the green light sensor and the lambda inverter system
BBa_K592017 Combined CcaS and CcaR with RBS before each
BBa_K592003 CcaR specific promoter, only allows transcription when CcaR-P is NOT present
BBa_K592012 Magenta pigment
BBa_KJ04450 RFP control
BBa_I746915 Superfolder GFP
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Exeter iGEM 2013 · Paint by Coli