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From 2013.igem.org

Contents 1 Organisation 2 Freiburg-Cooperation 3 Sponsoring 4 NRPS-Software 5 Lab 6 milestones 7 human practise 8 next meeting

Organisation

Hanna is new in the team as an advisor and will support us during the project. We have to book hotel and train tickets for her.

Freiburg-Cooperation

Three Freiburg team mmbers have been to Heidelberg monday/tuesday for cooperation. We did a Gibson Assembly with their constructs and transformed e. coli via electroporation and chemical transformation. After having dinnner they left the next day.

Sponsoring

• Klaus-Tschira-Stiftung supports us
• eppendorf supports us with consumables and hardware to lend

NRPS-Software

plan for web-interface and software/database-structure

• webinterface is ready to feed the database with details to single NRPSs; we will provide the software with about 10-20 NRPS pathways.
• getting the single domains is time consuming. automatization?
• -> tool to connect Pfam, clustalO and NCBI to automatically get domain borders for the databas!

Lab

• delftibacin
  • DelH is amplified; cloning using gibson approach after reassemlby of backbone
  • Del-rest: single parts are missing
• indigoidine
  • first Streptomyces strain didn't carry our gene of interest; new strain arrived today
  • construct with exchangeable PPTases (gibson or restriction)
• tyrocidine
  • module shuffling to test concept of modularity
  • bacterial strain is there, primer will be there next week
  • almost no restriction sites for RFC25

milestones

• DelH is few days in delay, backbone causes trouble
• DelRest is somewhat in plan
• Indigoidine is two weeks in delay but no other project part is influenced since we decided to start the tyrocidine part in parallel
• Tyrocidine; few days in delay because of primer shipment
• iGEM42; part time work is sufficient as expected
• NRPS-library; milestones need to be set; until now in time
• genomic integration; back-up strategy to clone the methylmalonyl-CoA-pathway on a plasmid to validate our delftibactin-e.coli-strain

human practise

• panel discussion
• life science lab, schools, essay on gold recovery
• discuss with NGOs about gold recovery, ecology and economy
• regional small jewelries, maybe for sponsoring
• interaction with lab-art project in summer
• publications in systembiologie.de, RNZ, Uni-Zeitung, ...
• don't put too much time in human practise

next meeting

with supervisors: tuesday July 30th; 5.30 pm