Team:Paris Saclay/Primers

From 2013.igem.org

Primers

Number Name Sequence 5' to 3' Organism
iPS33 pfnr_up ATATGAATTCGCGGCCGCTTCTAGAGT E.coli
iPS34 pfnr_down ATATCTGCAGCGGCCGCTACTAG

TAGTTCTTCGCTATTGAGAATAACATG

E.coli
iPS35 bphR2_up ATATGAATTCGCGGCCGCTTCTAGA

TGGAACTGCATGACCTGGA

P. pseuoalcaligenes
iPS36 bphR2_down ATATCTGCAGCGGCCGCTACTAGTAT

CAATCCGAAAACAGCTCGA

P. pseuoalcaligenes
iPS37 bphR1_up ATATGAATTCGCGGCCGCTTCTAGAG

ATGTCCCTGGGTGACATGCGTG

P. pseuoalcaligenes
iPS38 bphR1_down ATATCTGCAGCGGCCGCTACTAGTAA

GCAGTCAATCGGCGGTAGCGC

P. pseuoalcaligenes
iPS39 bphA1_up ATATGAATTCGCGGCCGCTTCTAGAGC

GCCAACATCCCACGTGTTTG

P. pseuoalcaligenes
iPS40 bphA1_down ATATCTGCAGCGGCCGCTACTAGTACC

CCCGGATCGCCTCCGGCGTC

P. pseuoalcaligenes
iPS41 SB-prep-3P-1 GCCGCTGCAGTCCGGCAAAAAA pSB1C3, E.coli
iPS42 SB-prep-2Ea TGCCACCTGACGTCTAAGAA pSB1C3, E.coli
iPS43 VF2 TGCCACCTGACGTCTAAGAA pSB1C3, E.coli
iPS44 VR ATTACCGCCTTTGAGTGAGC pSB1C3, E.coli
iPS45 Prefix-prom_nirB TCATGAATTCGCGGCCGCTTCTAGA

GCTACGTGCATCTATTTCTAT

E.coli
iPS46 suffixe-prom_nirB TACTCTGCAGCGGCCGCTACTAGTA

TGCCTCGATTTCTTTTCTAT

E.coli
iPS47 Prefix-prom_narK TCATGAATTCGCGGCCGCTTCTAGAG

ACATCGGTAAGGGTAGGGAT

E.coli
iPS48 suffixe-prom_narK TACTCTGCAGCGGCCGCTACTAGTAA

GACACCTCTGATACTCGTT

E.coli
iPS49 prefix-prom_narG TCATGAATTCGCGGCCGCTTCTAGAG

GGAATTTACTTTATTTTTCA

E.coli
iPS50 suffixe-prom_narG TACTCTGCAGCGGCCGCTACTAGTAT

TCTCCTGTGGGAGCCTGTC

E.coli
iPS51 Prefix from pSB1C3 CTCTAGAAGCGGCCGCGAATTC pSB1C3, E.coli
iPS52 sufix from pSB1C3 GTACCTGCAGCGGCCGCTACTAGTA pSB1C3,E.coli
iPS53 prefix-RBS-AmilCP CATAGAATTCGCGGCCGCTTCTAGAGAAAGAGG

AGAAATACTAGATGAGTGTGATCGCTAAACAAAT

pSB1C3,E.coli
iPS54 Bba_BphR2-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGATGGAA

CTGCATGACCTGGAT

P. pseuoalcaligenes
iPS55 Bba_BphR2-part1-Gibson-Reverse CAGTTCGTCCTGTAGTGCGG P. pseuoalcaligenes
iPS56 Bba_BphR2-part2-Gibson-Forward CACCGCACTACAGGACGAAC P. pseuoalcaligenes
iPS57 Bba_BphR2-part2-Gibson-Reverse CTGCAGCGGCCGCTACTAGTATCAATCCG

AAAACAGCTCGAACA

P. pseuoalcaligenes
iPS58 Bba_RBS-BphR2-part1-Gibson-Forward GAATTCGCGGGCCGCTTCTAGAGAAAGAGG

AGAAATACTAGATGGAACTGCATGACCTGGAT

P. pseuoalcaligenes
iPS59 Bba_FNR-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGATGATCCC

GGAAAAGCGAAT

E.coli
iPS60 Bba_FNR-part1-Gibson-Reverse CGGAACTCACGAGGGGAGAA E.coli
iPS61 Bba_FNR-part2-Gibson-Forward TTCTCCCCTCGTGAGTTCCG E.coli
iPS62 Bba_FNR-part2-Gibson-Reverse CTGCAGCGGCCGCTACTAGTATCAGGCAAC

GTTACGCGTAT

E.coli
iPS63 Bba_RBS-FNR-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGAAAGAGGA

GAAATACTAGATGATCCCGGAAAAGCGAATT

E.coli
iPS64 Suffix-from-pSB1C3-Gibson TACTAGTAGCGGCCGCTGCAG pSB1C3,E.coli
iPS65 Prefix-from-pSB1C3-Gibson CTCTAGAAGCGGCCGCGAATTC pSB1C3,E.coli