Team:TU-Delft/Notebook/2013/08/13/

From 2013.igem.org

Notebook

June
SunMonTueWedThuFriSat
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30
July
SunMonTueWedThuFriSat
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31










August
SunMonTueWedThuFriSat
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
September
SunMonTueWedThuFriSat
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30













13th of August


Lab work

1. Made Minimal media and starvation media for Bacillus subtilis.
2. Carried out colony PCR on BBConst-AgrAC-pP2-GFP.
3. Transformations of the pT7-peptides and pSB1C3-Magainin was carried out in XL1 Blue cells.
4. The oligopeptides and primers were PCRed and ligated with His-SUMO in pET23B backbone.