Team:TU-Delft/Notebook/2013/08/16/

From 2013.igem.org

Notebook

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16th of August


Lab work

1. pTet-RBS and pBAD-AgrAC-TT-pP2 were treated with CIP for 1-2 hours.
2. Transformations were carried out for pT7:lysis device ligation and pConst:AIPsensor:GFP:TT ligation done on 15/08/13.
3. Ligation of pTet and cI was carried out again.
7. pT7 and lysis device was ligated and kept in water bath at 16°C.