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Team:TU-Delft/Notebook/2013/09/02/
From 2013.igem.org
Notebook
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2nd of September
Lab work
1. Made glycerol stock of:
pT7 SUMO Maximin H5 pSB1C3
pT7 SUMO Signiferin pSB1C3
pT7 SUMO Magainin II pSB1C3
pT7 SUMO Maximin H5 Tet R (Bharat)
pT7 SUMO Signiferin Tet R (Bharat)
pT7 SUMO Magainin II Tet R (Bharat)
pT7 SUMO Maximin H5 Tet R (Derk)
pT7 SUMO Signiferin Tet R (Derk)
pT7 SUMO Magainin II Tet R (Derk)
pBAD Receiver Impure
pBAD Receiver Pure
pcI:Ulp
2. Did Colony PCR on the following:
pT7 SUMO Maximin H5 pSB1C3
pT7 SUMO Signiferin pSB1C3
pT7 SUMO Magainin II pSB1C3
pT7 SUMO Maximin H5 Tet R (Bharat)
pT7 SUMO Signiferin Tet R (Bharat)
pT7 SUMO Magainin II Tet R (Bharat)
pT7 SUMO Maximin H5 Tet R (Derk)
pT7 SUMO Signiferin Tet R (Derk)
pT7 SUMO Magainin II Tet R (Derk)
pBAD Receiver Impure
pBAD Receiver Pure
3. Did Mini prep on the following :
pT7 (BBa_I712074)
Lysis Device
4. Restriction digestion was carried out on:
Lysis device with E+X, X+P
pcI:Ulp: with E+S
pT7 with S+P
pTET:cI:TT with E+S
