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Team:TU-Delft/Notebook/2013/09/09/
From 2013.igem.org
Notebook
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9th of September
Lab work
1. Ran the restriction digestions on Gel:
pcI Ulp Lysis B 1 (E+X)
pcI Ulp Lysis B 2 (E+X)
pBAD Ulp TT (E+S)
2. Did Colony PCR on the following:
pT7 Lysis Batch C 1--6
pcI Ulp Lysis Batch C 1--6
The colony PCR was not very convincing and we discarded this batch.
3. Did Mini prep on the following :
pT7 lysis Batch C 1--6
pcI Ulp lysis Batch C 1--6
4. Restriction digestion was carried out on:
pBAD with S+P
AIP Receiver with X+P
5. Ligation was carried out on:
pBAD Ulp TT + pSB1C3
pBAD Ulp TT + pT7 SUMO Peptides
pcI Ulp Lysis + pTet cI TT
