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Team:TU-Delft/Notebook/2013/09/10/
From 2013.igem.org
Notebook
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10th of September
Lab work
1. Ran the restriction digestions on Gel:
pBAD Undigested
pBAD Digested (S+P)
pBAD (S+P)
AIP(X+P)
2. Did Colony PCR on the following:
Lysis pSB1C3 col 1
Lysis pSB1C3 col 2
Lysis Device Control
pcI Ulp Lysis Batch B
3. Inoculated on the following :
pBAD
AIP Receiver
AM124 SUMO in BL21
BBa_I746008 for pSB2K3 Backbone
4. Transformation was carried out on:
pT7 SUMO peptides --> BL21
pBAD Ulp TT pT7 SUMO Peptides --> TOP10
pBAD Ulp TT pSB1C3--> TOP10
pcI Ulp Lysis pTet cI TT --> TOP10
5. Gel purification and Ligation was carried out on:
pBAD + Receiver
6. PCR was carried out on pT7 SUMO Peptides Tet R to remove the pT7. PCR Purification was done on the above. Checked On gel:
