Team:TU-Delft/Notebook/2013/09/12/
From 2013.igem.org
Notebook
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12th of September
Lab work
1. Restriction digestions was carried out on and ran on gel:
pBAD with E+S
Lysis device with E+X and X+P
pcI Ulp with S+P and E+S
AIP Receiver with E+X
2. Did Colony PCR on the following:
pT7 Magainin His 6 in BL21
pT7 Signiferin His 6 in BL21
pT7 Lysis in BL21
3. Inoculated on the following :
pBAD Ulp TT Col 1 and Col 2
pcI Ulp Lysis pTet cI TT Col 2
pBAD Ulp TT pT7 SUMO Magainin Col 1 and col 2
pBAD Ulp TT pT7 SUMO Maximin Col 4 and col 5
pT7 Lysis B Plate 1
pT7 Lysis B Plate 1 in BL21