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Team:TU-Delft/Notebook/2013/09/13/
From 2013.igem.org
Notebook
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13th of September
Lab work
1. Did Colony PCR was done on pBAD Receiver :
3. Mini prep was done on the following :
pBAD Ulp TT Col 1 and Col 2
pBAD Ulp TT pT7 SUMO Magainin Col 1 and col 2
pBAD Ulp TT pT7 SUMO Maximin Col 4 and col 5
4. Ligation was carried out on:
pBAD (Insert) + Receiver (Vector)
pcI Ulp (Vector) + Lysis (Insert)
pcI Ulp (Insert) + Lysis (Vector)
pBAD Ulp TT (Insert) + pT7 SUMO Signiferin (Vector) --> TOP10
5. Transformation was done on the above ligations.
6. MIC experiment was done for the Synthetic peptides Magainin II and Maximin H5.
