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Team:TU-Delft/Notebook/2013/09/18/
From 2013.igem.org
Notebook
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18th of September
Lab work
1. Restriction digestion was carried out on:
BBa_P0440 to get pSB1C3 with E+S
Lysis Devise with E+S
pTet cI TT with E+S
pcI Ulp with E+S
2. Transformations in Xl 1 Blue cells were done for :
pBAD Receiver + SUMO peptide Tet R
Lysis device + pSB1C3
pBAD Ulp TT + pT7 SUMO Signiferin
3. Reinoculated pT7 Lysis in BL21 plysS for lysis experiment on plate reader.
