Team:UNITN-Trento/Notebook/Labposts/06/60

From 2013.igem.org

{ "date" : "2013-06-26", "author" : "emil-gabriele", "title" : "Ligation and transformation of R0010 + Sam sinthethase", "content" : "Today we added 1µl of SAP to the o/n digestion and then incubated it for 1.5h.Then we performed the ligation of SAMynthetase (previously digested with XbaI and PstI), following the ligation protocol.

Ligation mixes
Ctrl1:11:3
Buffer2.5µl
plasmid5.73µl
insert03.09µl9.27µl
ligase1µl
water15.77µl12.68µl6.50µl
Afterwards, Gabriele purified the product following the usual purification protocol and then transformed it in NEB10β cells following the transformation protocol.

Gabriele prepared also 4 inocula from the pSB1C3+R0010 CM-plate, just to be sure that it is (not) there.", "tags" : "Plac-SAMsynthetase" }