{"date" : "2013-07-30","author" : "thomas","title" : "Bacillus transformation!!!","content" : "In order to test the transformation protocol for B. subtilis that Groeningen team kindly sent to us I prepared a competent cell Media following this protocol. I proceeded then perfonming the transformation using the empty vector BBa_K823026. I finally plated the tranformed cells into LB agar plates with 10 ug/ml of Kanamycin. The day after took a picture of the plate and performed a Gram staining following this protocol.

As you can see from the pictures, seems that Bacillus was successful transformed!!!!","tags" : "b. subtilis-pspac"}