Team:USTC CHINA/Notebook/Protocols/Ligation

From 2013.igem.org

Ligation

Performed with TaKaRa Ligation Kit (Solution I)
1. System

Volume (μl)
Solution I 5
DNA (fragment + carrier) 5

fragment : carrier (radio in mol)= 3:1~10:1
carrier≥0.03 pmol
2. Gently vortex the samples and spin down.
3. Incubate at 16°C in a low temperature water thermostat for at least 3h.