Team:UT Dallas/week1

From 2013.igem.org

7/5/2013

Transformation

[GFP] Kit plate 5. Well 14k. Backbone pSB1A2. Resistance A. i5-5-1

[LacI] Kit plate 5. Well 20. Backbone pSB1A2. Resistance A. i5-5-2

7/6/2013

Colony Picking

I5-5-1 →  2 colonies → picked 2 → i5-5-3

                                        → i5-5-4

I5-5-2 → 0 colonies

7/7/2013

Mini Prep

I5-5-3: 67.4 ng/ul        [GFP]

I5-5-4: 98.7 ng/ul        [GFP]

I5-5-5: 408.7 ng/ul        [+ confiol]

Test Digestion

<a href="#" name="d2fd616f7186be08d9c7705395c2136d5ae85998"></a><a href="#" name="0"></a><tbody></tbody>

I5-5-31

I5-5-42

DNA

4

2

BSA

1

1

Buffer 4

1

1

EcoRI

1

1

Pst

1

1

H2O

2

4

10

10