Protocols

• 3A assembly cloning cycle
• Agarose gel electrophoresis
• Autoclaver manual
• BioBrick assembly manual
• CCMB80 buffer protocol
• Competent cell preparation
• Competitiontest
• High pressure liquid chromatography
• LB and LB-agar protocol
• M17 medium preparation, Lactococcus lactis
• Overnight culture and glycerol stock
• Phusion PCR Blank protocol
• Plasmidstability
• Preparation of stocks for Lactococcus lactis
• Preparation of cells Westernblot
• Spectrophotometry of tyrosine ammonia lyase
• TBE-buffer protocol
• Transformation by Electroporation for L.reuteri
• Transforming e-coli protocol
• WesternBlot