LOCUS       BBa_K1150020            7485 bp    DNA     circular UNA 23-OCT-2013
DEFINITION  uniCAS Activator, Freiburg 2013.
ACCESSION   urn.local...1382991826956.26
VERSION     urn.local...1382991826956.26
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..580
                     /note="Geneious type: Promoter"
                     /label="CMV"
     source          <583..595
                     /label="Präfix"
     misc_feature    587..4756
                     /note="Geneious type: dCas9 [RFC 25]"
                     /label="dCas9 [RFC 25]"
     misc_feature    590..595
                     /note="Geneious type: Prefix/Suffix"
                     /label="Prefix"
     misc_feature    596..622
                     /note="Geneious type: Short Element"
                     /label="HA"
     source          623
                     /label="Suffix"
     source          <624..628
                     /label="Präfix"
     misc_feature    629..649
                     /note="Geneious type: Short Element"
                     /label="NLS 1"
     source          650
                     /label="Suffix"
     source          <651..655
                     /label="Präfix"
     misc_feature    3170..3172
                     /note="Geneious type: Mutation"
                     /label="H840A"
     misc_feature    4757..4777
                     /note="Geneious type: Short Element"
                     /label="Linker"
     source          4757
                     /label="Suffix"
     misc_feature    4778..5185
                     /note="Geneious type: Effector"
                     /label="VP16 [RFC 25]"
     source          <5151..5155
                     /label="Präfix"
     misc_feature    5155^5156
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    5156..5176
                     /note="Geneious type: Short Element"
                     /label="NLS 2"
     misc_feature    5177..5182
                     /note="Geneious type: Prefix/Suffix"
                     /label="Suffix"
     source          5177..>5187
                     /label="Suffix"
     terminator      5194..5415
                     /note="Geneious type: Terminator"
                     /label="BgH"
     misc_feature    5417..5436
                     /note="Geneious type: Prefix/Suffix"
                     /label="Suffix"
     source          5436
                     /label="Suffix"
     misc_feature    5676..6290
                     /note="Geneious type: Backbone"
                     /label="Replicon"
     misc_feature    6575..7234
                     /note="Geneious type: Backbone"
                     /label="Chloramphenicol resistance"
     misc_feature    7464..7484
                     /note="Geneious type: Prefix/Suffix"
                     /label="Prefix"
     source          7464
                     /label="Präfix"
ORIGIN      
        1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
       61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagatgg ccggctaccc
      601 atacgatgtt ccagattacg ctaccggccc tgcaagaaaa agattgaata ccggcgacaa
      661 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga
      721 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
      781 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
      841 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
      901 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
      961 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
     1021 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     1081 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     1141 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     1201 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     1261 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     1321 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc
     1381 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     1441 acttcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     1501 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     1561 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     1621 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     1681 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggatacat
     1741 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     1801 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     1861 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     1921 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     1981 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     2041 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     2101 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     2161 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     2221 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     2281 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     2341 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     2401 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     2461 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     2521 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     2581 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     2641 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     2701 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     2761 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     2821 cattgccaat ctggcaggca gccccgccat taagaagggc atcctgcaaa cagtgaaggt
     2881 ggtggacgag ctcgtgaaag tgatgggacg gcacaagccc gagaacatcg tgatcgaaat
     2941 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     3001 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     3061 cacccagctt cagaacgaga agctgtacct gtactacctg caaaatgggc gggatatgta
     3121 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc
     3181 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
     3241 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     3301 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
     3361 cgagagaggc ggcctgagcg aactggataa ggccggwttc atcaagagac agctggtgga
     3421 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     3481 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     3541 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     3601 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     3661 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     3721 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     3781 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     3841 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
     3901 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     3961 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
     4021 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
     4081 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     4141 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     4201 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     4261 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gcgggcgaac tgcaaaaggg
     4321 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     4381 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     4441 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     4501 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     4561 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
     4621 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
     4681 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     4741 tcagctggga ggcgacaccg gcgcaggctc caccggcgcg cgtacgaaaa acaattacgg
     4801 gtctaccatc gagggcctgc tcgatctccc ggacgacgac gcccccgaag aggcggggct
     4861 ggcggctccg cgcctgtcct ttctccccgc gggacacacg cgcagactgt cgacggcccc
     4921 cccgaccgat gtcagcctgg gggacgagct ccacttagac ggcgaggacg tggcgatggc
     4981 gcatgccgac gcgctagacg atttcgatct ggacatgttg ggggacgggg attccccggg
     5041 tccgggattt accccccacg actccgcccc ctacggcgct ctggatatgg ccgacttcga
     5101 gtttgagcag atgtttaccg atgcccttgg aattgacgag tacggtggga ccggccctgc
     5161 aagaaaaaga ttgaataccg gttaatacta gaggtgcctt ctagttgcca gccatctgtt
     5221 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5281 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5341 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     5401 gcggtgggct ctatgtacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc
     5461 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc
     5521 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5581 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5641 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
     5701 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5761 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5821 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5881 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5941 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6001 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6061 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6121 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6181 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6241 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6301 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6361 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6421 tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc
     6481 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     6541 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
     6601 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     6661 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     6721 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     6781 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
     6841 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     6901 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     6961 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     7021 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     7081 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     7141 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     7201 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     7261 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     7321 ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg
     7381 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     7441 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagag
//
