LOCUS       BBa_K1150029            8223 bp    DNA     circular UNA 24-OCT-2013
DEFINITION  uniCAS UV Light Stimulator, Freiburg 2013.
ACCESSION   urn.local...1382991827153.35
VERSION     urn.local...1382991827153.35
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..580
                     /note="Geneious type: Promoter"
                     /label="CMV"
     source          <583..595
                     /label="Präfix"
     misc_feature    587..4756
                     /note="Geneious type: dCas9 [RFC 25]"
                     /label="dCas9 [RFC 25]"
     misc_feature    590..595
                     /note="Geneious type: Prefix/Suffix"
                     /label="Prefix"
     misc_feature    596..622
                     /note="Geneious type: Short Element"
                     /label="HA"
     source          623
                     /label="Suffix"
     source          <624..628
                     /label="Präfix"
     misc_feature    629..649
                     /note="Geneious type: Short Element"
                     /label="NLS 1"
     source          650
                     /label="Suffix"
     source          <651..655
                     /label="Präfix"
     misc_feature    3170..3172
                     /note="Geneious type: Mutation"
                     /label="H840A"
     source          4757
                     /label="Suffix"
     misc_feature    4758..4776
                     /note="Geneious type: Short Element"
                     /label="Linker"
     misc_feature    4778..5923
                     /note="Geneious type: Light Part"
                     /label="UVR8 [RFC 25]"
     misc_feature    5894..5914
                     /note="Geneious type: Short Element"
                     /label="NLS 2"
     misc_feature    5915..5920
                     /note="Geneious type: Prefix/Suffix"
                     /label="Suffix"
     source          5915..>5925
                     /label="Suffix"
     terminator      5932..6153
                     /note="Geneious type: Terminator"
                     /label="BgH"
     misc_feature    6155..6174
                     /note="Geneious type: Prefix/Suffix"
                     /label="Suffix"
     source          6174
                     /label="Suffix"
     misc_feature    6414..7028
                     /note="Geneious type: Backbone"
                     /label="Replicon"
     misc_feature    7313..7972
                     /note="Geneious type: Backbone"
                     /label="Chloramphenicol resistance"
     misc_feature    8202..8222
                     /note="Geneious type: Prefix/Suffix"
                     /label="Prefix"
     source          8202
                     /label="Präfix"
ORIGIN      
        1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
       61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagatgg ccggctaccc
      601 atacgatgtt ccagattacg ctaccggccc tgcaagaaaa agattgaata ccggcgacaa
      661 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga
      721 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
      781 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
      841 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
      901 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
      961 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
     1021 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     1081 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     1141 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     1201 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     1261 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     1321 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc
     1381 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     1441 acttcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     1501 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     1561 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     1621 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     1681 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggatacat
     1741 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     1801 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     1861 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     1921 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     1981 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     2041 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     2101 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     2161 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     2221 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     2281 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     2341 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     2401 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     2461 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     2521 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     2581 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     2641 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     2701 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     2761 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     2821 cattgccaat ctggcaggca gccccgccat taagaagggc atcctgcaaa cagtgaaggt
     2881 ggtggacgag ctcgtgaaag tgatgggacg gcacaagccc gagaacatcg tgatcgaaat
     2941 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     3001 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     3061 cacccagctt cagaacgaga agctgtacct gtactacctg caaaatgggc gggatatgta
     3121 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc
     3181 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
     3241 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     3301 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
     3361 cgagagaggc ggcctgagcg aactggataa ggccggwttc atcaagagac agctggtgga
     3421 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     3481 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     3541 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     3601 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     3661 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     3721 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     3781 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     3841 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
     3901 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     3961 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
     4021 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
     4081 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     4141 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     4201 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     4261 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gcgggcgaac tgcaaaaggg
     4321 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     4381 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     4441 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     4501 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     4561 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
     4621 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
     4681 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     4741 tcagctggga ggcgacaccg gcgcaggctc caccggcgct cctcctcgta aggttcttat
     4801 catctccgct ggtgctagcc actccgtcgc tcttctctct ggtgacattg tttgttcttg
     4861 gggtcgagga gaggatggac agttaggtca tggcgatgca gaggatcgac cttctccgac
     4921 tcagcttagc gctttagatg gccaccaaat tgtttccgtt acctgtggtg ctgatcacac
     4981 tgttgcttat tcacaatcag gcatggaagt ctacagttgg ggatggggtg attttgggag
     5041 attaggccat ggtaactcaa gcgacttgtt tactccgcta ccaatcaaag cattgcacgg
     5101 tattcggatc aagcagattg cttgtgggga tagtcattgt ttggctgtca ctatggaagg
     5161 agaggtccag agttggggcc gcaaccagaa tggtcaactt ggtctggggg acaccgaaga
     5221 ttctctagtg cctcagaaga ttcaagcctt tgagggaata cgaatcaaaa tggttgctgc
     5281 tggtgcagaa cacactgctg ccgttacaga agatggtgac ctctatggat ggggctgggg
     5341 aagatacgga aatttgggat taggtgaccg gactgaccgc ttagttcctg aaagagttac
     5401 ctctactggt ggtgagaaaa tgtcaatggt tgcttgtgga tggcggcaca caatatcagt
     5461 ttcctactct ggagcattgt atacttatgg atggagcaaa tatggacagc taggacatgg
     5521 agacttggag gatcacctta ttcctcacaa actggaagca ctgagcaaca gttttatctc
     5581 ccagatttcg ggaggttgga gacatacaat ggcattgact tcagatggaa aactatatgg
     5641 atggggttgg aataagtttg gacaagtagg agtcggcaat aatttagatc agtgttctcc
     5701 tgtgcaagtg cgatttcccg atgatcagaa agtagttcaa gtctcatgtg gatggagaca
     5761 taccttggct gtcactgaaa gaaataacgt gtttgcttgg ggtagaggta caaatggaca
     5821 gctcggcatt ggagagtcgg ttgacaggaa ctttcccaag attatagagg cactcagcgt
     5881 cgatggaacc ggccctgcaa gaaaaagatt gaataccggt taatactaga ggtgccttct
     5941 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6001 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     6061 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     6121 agcaggcatg ctggggatgc ggtgggctct atgtactagt agcggccgct gcagtccggc
     6181 aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
     6241 cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6301 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6361 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6421 ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6481 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6541 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6601 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6661 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6721 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6781 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6841 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6901 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6961 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7021 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7081 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7141 ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt
     7201 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     7261 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
     7321 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     7381 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     7441 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     7501 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
     7561 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     7621 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     7681 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     7741 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     7801 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     7861 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     7921 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     7981 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     8041 tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa
     8101 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc
     8161 agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta
     8221 gag
//
