LOCUS       pSB3K3+BBa_J04450       3854 bp                         05-SEP-2013
EMBL_ID     pSB3K3+BBa_J04450       3854 bp                         05-SEP-2013
UNIMARK     Annotations
            pSB3K3+BBa_J04450
FEATURES             Location/Qualifiers
     source          1..3854
                     /comment="Molecule is created with Unipro UGENE v1.12"
     misc_feature    2..73
                     /gene="E. coli his operon terminator"
                     /ugene_name="misc"
     stem_loop       10..44
                     /label="terminator stem loop"
     misc_feature    14..15
                     /gene="deletion of two bases"
                     /ugene_name="misc"
     primer_bind     complement(137..156)
                     /label="VR"
     CDS             complement(219..1034)
                     /gene="kanR (rev)"
     CDS             complement(1366..2190)
                     /gene="p15A ORI (rev)"
     primer_bind     2591..2610
                     /label="VF2"
     misc_feature    complement(2667..2670)
                     /gene="end of terminator"
                     /ugene_name="misc"
     stem_loop       complement(2680..2699)
                     /label="terminator stem loop"
     misc_feature    2731..2930
                     /label="BBa_R0010"
     misc_binding    2819..2856
                     /label="CAP"
     promoter        2867..2872
                     /label=-35
     promoter        2891..2896
                     /label=-10
     misc_binding    2896..2930
                     /label="LacI"
     misc_feature    2903..2903
                     /label="start: "
     misc_feature    complement(2913..2962)
                     /mismatches=0
                     /ugene_name="RB63_rv"
                     /ugene_group="Primer"
     misc_feature    2943..2986
                     /mismatches=0
                     /ugene_name="RB35_fw"
                     /ugene_group="Primer"
     misc_feature    2960..3634
                     /mismatches=0
                     /ugene_name="sfp"
                     /ugene_group="gene"
     misc_feature    complement(3611..3654)
                     /mismatches=0
                     /ugene_name="RB36_rv"
                     /ugene_group="Primer"
     misc_feature    3655..3672
                     /label="BBa_E1010"
     misc_feature    3655..3689
                     /mismatches=0
                     /ugene_name="RB21_fw"
                     /ugene_group="Primer"
     misc_feature    3673..3697
                     /label="barcode: "
     misc_feature    3706..3785
                     /label="BBa_B0010"
     stem_loop       3717..3760
     misc_feature    3794..3834
                     /label="BBa_B0012"
     stem_loop       3801..3820
                     /label="T7 TE"
     polyA_signal    3821..3834
     misc_feature    3827..3827
                     /label="stop: "
ORIGIN
        1 GTCCGGCAAA AAAACGGGCA AGGTGTCACC ACCCTGCCCT TTTTCTTTAA AACCGAAAAG
       61 ATTACTTCGC GTTATGCAGG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
      121 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATCTCG AGTCCCGTCA AGTCAGCGTA
      181 ATGCTCTGCC AGTGTTACAA CCAATTAACC AATTCTGATT AGAAAAACTC ATCGAGCATC
      241 AAATGAAACT GCAATTTATT CATATCAGGA TTATCAATAC CATATTTTTG AAAAAGCCGT
      301 TTCTGTAATG AAGGAGAAAA CTCACCGAGG CAGTTCCATA GGATGGCAAG ATCCTGGTAT
      361 CGGTCTGCGA TTCCGACTCG TCCAACATCA ATACAACCTA TTAATTTCCC CTCGTCAAAA
      421 ATAAGGTTAT CAAGTGAGAA ATCACCATGA GTGACGACTG AATCCGGTGA GAATGGCAAA
      481 AGCTTATGCA TTTCTTTCCA GACTTGTTCA ACAGGCCAGC CATTACGCTC GTCATCAAAA
      541 TCACTCGCAT CAACCAAACC GTTATTCATT CGTGATTGCG CCTGAGCGAG ACGAAATACG
      601 CGATCGCTGT TAAAAGGACA ATTACAAACA GGAATCGAAT GCAACCGGCG CAGGAACACT
      661 GCCAGCGCAT CAACAATATT TTCACCTGAA TCAGGATATT CTTCTAATAC CTGGAATGCT
      721 GTTTTCCCGG GGATCGCAGT GGTGAGTAAC CATGCATCAT CAGGAGTACG GATAAAATGC
      781 TTGATGGTCG GAAGAGGCAT AAATTCCGTC AGCCAGTTTA GTCTGACCAT CTCATCTGTA
      841 ACATCATTGG CAACGCTACC TTTGCCATGT TTCAGAAACA ACTCTGGCGC ATCGGGCTTC
      901 CCATACAATC GATAGATTGT CGCACCTGAT TGCCCGACAT TATCGCGAGC CCATTTATAC
      961 CCATATAAAT CAGCATCCAT GTTGGAATTT AATCGCGGCC TCGAGCAAGA CGTTTCCCGT
     1021 TGAATATGGC TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG TTTTATTGTT
     1081 CATGATGATA TATTTTTATC TTGTGCAATG TAACATCAGA GATTTTGAGA CACAACGTGG
     1141 CTTTGTTGAA TAAATCGAAC TTTTGCTGAG TTGAAGGATC AGATCACGCA TCTTCCCGAC
     1201 AACGCAGACC GTTCCGTGGC AAAGCAAAAG TTCAAAATCA CCAACTGGTC CACCTACAAC
     1261 AAAGCTCTCA TCAACCGTGG CTCCCTCACT TTCTGGCTGG ATGATGGGGC GATTCAGGCC
     1321 TGGTATGAGT CAGCAACACC TTCTTCACGA GGCAGACCTC AGCGCTAGCG GAGTGTATAC
     1381 TGGCTTACTA TGTTGGCACT GATGAGGGTG TCAGTGAAGT GCTTCATGTG GCAGGAGAAA
     1441 AAAGGCTGCA CCGGTGCGTC AGCAGAATAT GTGATACAGG ATATATTCCG CTTCCTCGCT
     1501 CACTGACTCG CTACGCTCGG TCGTTCGACT GCGGCGAGCG GAAATGGCTT ACGAACGGGG
     1561 CGGAGATTTC CTGGAAGATG CCAGGAAGAT ACTTAACAGG GAAGTGAGAG GGCCGCGGCA
     1621 AAGCCGTTTT TCCATAGGCT CCGCCCCCCT GACAAGCATC ACGAAATCTG ACGCTCAAAT
     1681 CAGTGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCT GGCGGCTCCC
     1741 TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT TTACCGGTGT CATTCCGCTG TTATGGCCGC
     1801 GTTTGTCTCA TTCCACGCCT GACACTCAGT TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC
     1861 TGTATGCACG AACCCCCCGT TCAGTCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     1921 GAGTCCAACC CGGAAAGACA TGCAAAAGCA CCACTGGCAG CAGCCACTGG TAATTGATTT
     1981 AGAGGAGTTA GTCTTGAAGT CATGCGCCGG TTAAGGCTAA ACTGAAAGGA CAAGTTTTGG
     2041 TGACTGCGCT CCTCCAAGCC AGTTACCTCG GTTCAAAGAG TTGGTAGCTC AGAGAACCTT
     2101 CGAAAAACCG CCCTGCAAGG CGGTTTTTTC GTTTTCAGAG CAAGAGATTA CGCGCAGACC
     2161 AAAACGATCT CAAGAAGATC ATCTTATTAA GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
     2221 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
     2281 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
     2341 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
     2401 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG
     2461 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
     2521 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
     2581 TATTCCATGG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA
     2641 AATAGGCGTA TCACGAGGCA GAATTTCAGA TAAAAAAAAT CCTTAGCTTT CGCTAAGGAT
     2701 GATTTCTGGA ATTCGCGGCC GCTTCTAGAG CAATACGCAA ACCGCCTCTC CCCGCGCGTT
     2761 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC
     2821 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC
     2881 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA TACTAGAGTT
     2941 CACACAGGAA ACCGGATCCA TGAAGATTTA CGGAATTTAT ATGGACCGCC CGCTTTCACA
     3001 GGAAGAAAAT GAACGGTTCA TGACTTTCAT ATCACCTGAA AAACGGGAGA AATGCCGGAG
     3061 ATTTTATCAT AAAGAAGATG CTCACCGCAC CCTGCTGGGA GATGTGCTCG TTCGCTCAGT
     3121 CATAAGCAGG CAGTATCAGT TGGACAAATC CGATATCCGC TTTAGCACGC AGGAATACGG
     3181 GAAGCCGTGC ATCCCTGATC TTCCCGACGC TCATTTCAAC ATTTCTCACT CCGGCCGCTG
     3241 GGTCATTGGT GCGTTTGATT CACAGCCGAT CGGCATAGAT ATCGAAAAAA CGAAACCGAT
     3301 CAGCCTTGAG ATCGCCAAGC GCTTCTTTTC AAAAACAGAG TACAGCGACC TTTTAGCAAA
     3361 AGACAAGGAC GAGCAGACAG ACTATTTTTA TCATCTATGG TCAATGAAAG AAAGCTTTAT
     3421 CAAACAGGAA GGCAAAGGCT TATCGCTTCC GCTTGATTCC TTTTCAGTGC GCCTGCATCA
     3481 GGACGGACAA GTATCCATTG AGCTTCCGGA CAGCCATTCC CCATGCTATA TCAAAACGTA
     3541 TGAGGTCGAT CCCGGCTACA AAATGGCTGT ATGCGCCGCA CACCCTGATT TCCCCGAGGA
     3601 TATCACAATG GTCTCGTACG AAGAGCTTTT ATAATGAGCT AGCTAATAAC GCTGTAATGA
     3661 GCTAGCTAAT AACGCTGATA GTGCTAGTGT AGATCGCTAC TAGAGCCAGG CATCAAATAA
     3721 AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG
     3781 CTCTCTACTA GAGTCACACT GGCTCACCTT CGGGTGGGCC TTTCTGCGTT TATATACTAG
     3841 TAGCGGCCGC TGCA
//
