LOCUS       new_mol1                6520 bp    CIRCULAR             18-SEP-2013
EMBL_ID     new_mol1                6520 bp    CIRCULAR             18-SEP-2013
UNIMARK     new_mol1 annotations
            new_mol1
FEATURES             Location/Qualifiers
     source          1..6520
                     /comment="Molecule is created with Unipro UGENE v1.12"
     stem_loop       16..87
                     /label="E. coli his operon terminator"
     stem_loop       24..58
                     /label="Stem loop"
     primer_bind     complement(151..170)
                     /label="Verification reverse (VR) primer binding site"
     misc_feature    complement(255..869)
                     /gene="rep (pMB1)"
                     /ugene_name="misc"
     misc_feature    270..270
                     /gene="ORI"
                     /ugene_name="misc"
     stem_loop       1036..1141
                     /label="T0 terminator"
     CDS             complement(1154..1813)
                     /gene="Chloramphenicol resistance"
     primer_bind     1927..1946
                     /label="Verification forward (VF2) primer binding site"
     stem_loop       complement(2003..2006)
                     /label="end of terminator"
     stem_loop       complement(2016..2035)
                     /label="Stem loop"
     misc_feature    2045..2064
                     /label="DNA: BioBrick prefix"
     misc_feature    2065..2264
                     /label="BBa_R0010"
     misc_binding    2153..2190
                     /label="CAP"
     promoter        2201..2206
                     /label=-35
     promoter        2225..2230
                     /label=-10
     misc_binding    2230..2264
                     /label="LacI"
     misc_feature    2237..2237
                     /label="start: "
     misc_feature    complement(2267..2322)
                     /mismatches=0
                     /ugene_name="IK35"
                     /ugene_group="Primer"
     misc_feature    2273..2284
                     /ugene_name="BBa_B0034"
     misc_feature    2277..2280
                     /label="Conserved: "
     misc_feature    2291..3883
                     /source="Streptomyces coelicolor A3(2)"
                     /ugene_name="pccB"
     misc_feature    2291..2309
                     /mismatches=0
                     /ugene_name="IK34"
                     /ugene_group="Primer"
     misc_feature    3884..5674
                     /source="Streptomyces coelicolor A3(2)"
                     /ugene_name="accA2"
     misc_feature    3905..3907
                     /locus_ref="accA2"
                     /ugene_name="alternate startcodon"
     misc_feature    5645..5729
                     /mismatches=0
                     /ugene_name="IK30"
                     /ugene_group="Primer"
     misc_feature    complement(5654..5685)
                     /mismatches=0
                     /ugene_name="IK29"
                     /ugene_group="Primer"
     misc_feature    5667..5667
                     /label="putative"
                     /ugene_name="insertion"
     misc_feature    5682..5696
                     /ugene_name="BBa_B0030"
     gene            5703..6377
                     /gene="sfp gene"
     CDS             5703..6377
                     /gene="sfp gene"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA44858.1"
                     /db_xref="GI:40139"
                     /db_xref="GOA:P39135"
                     /db_xref="InterPro:IPR004568"
                     /db_xref="InterPro:IPR008278"
                     /db_xref="PDB:1QR0"
                     /db_xref="PDB:2GE0"
                     /db_xref="PDB:2GE1"
                     /db_xref="UniProtKB/Swiss-Prot:P39135"
                     /translation="MKIYGIYMDRPLSQEENERFMTFISPEKREKCRRFYHKEDAHRT
                     LLGDVLVRSVISRQYQLDKSDIRFSTQEYGKPCIPDLPDAHFNISHSGRWVIGAFDSQ
                     PIGIDIEKTKPISLEIAKRFFSKTEYSDLLAKDKDEQTDYFYHLWSMKESFIKQEGKG
                     LSLPLDSFSVRLHQDGQVSIELPDSHSPCYIKTYEVDPGYKMAVCAAHPDFPEDITMV
                     SYEELL"
     misc_feature    6345..6404
                     /mismatches=0
                     /ugene_name="IK32"
                     /ugene_group="Primer"
     misc_feature    6386..6465
                     /label="BBa_B0010"
     stem_loop       6397..6440
     misc_feature    6474..6514
                     /label="BBa_B0012"
     stem_loop       6481..6500
                     /label="T7 TE"
     polyA_signal    6501..6514
     misc_feature    6507..6507
                     /label="stop: "
     misc_feature    join(6515..6520,1..15)
                     /label="DNA: BioBrick suffix"
ORIGIN
        1 TAGCGGCCGC TGCAGTCCGG CAAAAAAGGG CAAGGTGTCA CCACCCTGCC CTTTTTCTTT
       61 AAAACCGAAA AGATTACTTC GCGTTATGCA GGCTTCCTCG CTCACTGACT CGCTGCGCTC
      121 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
      181 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
      241 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCACAGGCTC CGCCCCCCTG ACGAGCATCA
      301 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
      361 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
      421 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
      481 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
      541 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
      601 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
      661 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
      721 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
      781 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
      841 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
      901 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
      961 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     1021 TGACAGCTCG AGGCTTGGAT TCTCACCAAT AAAAAACGCC CGGCGGCAAC CGAGCGTTCT
     1081 GAACAAATCC AGATGGAGTT CTGAGGTCAT TACTGGATCT ATCAACAGGA GTCCAAGCGA
     1141 GCTCGATATC AAATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA
     1201 GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG AACCTGAATC GCCAGCGGCA
     1261 TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT
     1321 TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA
     1381 CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG
     1441 CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA
     1501 GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC
     1561 ATATCACCAG CTCACCGTCT TTCATTGCCA TACGAAATTC CGGATGAGCA TTCATCAGGC
     1621 GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA
     1681 AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA
     1741 ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA
     1801 TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGATAACTCA AAAAATACGC
     1861 CCGGTAGTGA TCTTATTTCA TTATGGTGAA AGTTGGAACC TCTTACGTGC CCGATCAACT
     1921 CGAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG
     1981 CGTATCACGA GGCAGAATTT CAGATAAAAA AAATCCTTAG CTTTCGCTAA GGATGATTTC
     2041 TGGAATTCGC GGCCGCTTCT AGAGCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
     2101 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
     2161 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
     2221 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACATACTAG AGAAAGAGGA
     2281 GAAATACTAG ATGTCCGAGC CGGAAGAGCA GCAGCCCGAC ATCCACACGA CCGCGGGCAA
     2341 GCTCGCGGAT CTCAGGCGCC GTATCGAGGA AGCGACGCAC GCCGGTTCCG CACGCGCCGT
     2401 CGAGAAGCAG CACGCCAAGG GCAAGCTGAC GGCTCGTGAA CGCATCGACC TCCTCCTCGA
     2461 CGAGGGTTCC TTCGTCGAGC TGGACGAGTT CGCCCGGCAC CGCTCCACCA ACTTCGGCCT
     2521 CGACGCCAAC CGCCCCTACG GCGACGGCGT CGTCACCGGC TACGGCACCG TCGACGGCCG
     2581 CCCCGTGGCC GTCTTCTCCC AGGACTTCAC CGTCTTCGGC GGCGCGCTGG GCGAGGTCTA
     2641 CGGCCAGAAG ATCGTCAAGG TGATGGACTT CGCCCTCAAG ACCGGCTGCC CGGTCGTCGG
     2701 CATCAACGAC TCCGGCGGCG CCCGCATCCA GGAGGGCGTG GCCTCCCTCG GCGCCTACGG
     2761 CGAGATCTTC CGCCGCAACA CCCACGCCTC CGGCGTGATC CCGCAGATCA GCCTGGTCGT
     2821 CGGCCCGTGT GCGGGCGGCG CGGTGTACTC CCCCGCGATC ACCGACTTCA CGGTGATGGT
     2881 GGACCAGACC AGCCACATGT TCATCACCGG TCCCGACGTC ATCAAGACGG TCACCGGCGA
     2941 GGACGTCGGC TTCGAGGAGC TGGGCGGCGC CCGCACCCAC AACTCCACCT CGGGCGTGGC
     3001 CCACCACATG GCCGGCGACG AGAAGGACGC GGTCGAGTAC GTCAAGCAGC TCCTGTCGTA
     3061 CCTGCCGTCC AACAACCTCT CCGAGCCCCC CGCCTTCCCG GAGGAGGCGG ACCTCGCGGT
     3121 CACGGACGAG GACGCCGAGC TGGACACGAT CGTCCCGGAC TCGGCGAACC AGCCCTACGA
     3181 CATGCACTCC GTCATCGAGC ACGTCCTGGA CGACGCCGAG TTCTTCGAGA CGCAACCCCT
     3241 CTTCGCGCCG AACATCCTCA CCGGCTTCGG CCGCGTGGAG GGCCGCCCGG TCGGCATCGT
     3301 CGCCAACCAG CCCATGCAGT TCGCCGGCTG CCTGGACATC ACGGCCTCCG AGAAGGCGGC
     3361 CCGCTTCGTG CGCACCTGCG ACGCCTTCAA CGTCCCCGTC CTCACCTTCG TGGACGTCCC
     3421 CGGCTTCCTG CCCGGCGTCG ACCAGGAGCA CGACGGCATC ATCCGCCGCG GCGCCAAGCT
     3481 GATCTTCGCC TACGCCGAGG CCACGGTGCC GCTCATCACG GTCATCACCC GCAAGGCCTT
     3541 CGGCGGCGCC TACGACGTCA TGGGCTCCAA GCACCTGGGC GCCGACCTCA ACCTGGCCTG
     3601 GCCCACCGCC CAGATCGCCG TCATGGGCGC CCAAGGCGCG GTCAACATCC TGCACCGCCG
     3661 CACCATCGCC GACGCCGGTG ACGACGCCGA GGCCACCCGG GCCCGCCTGA TCCAGGAGTA
     3721 CGAGGACGCC CTCCTCAACC CCTACACGGC GGCCGAACGC GGCTACGTCG ACGCCGTGAT
     3781 CATGCCCTCC GACACTCGCC GCCACATCGT CCGCGGCCTG CGCCAGCTGC GCACCAAGCG
     3841 CGAGTCCCTG CCCCCGAAGA AGCACGGCAA CATCCCCCTG TGAATGCATG AGGGAGCCTC
     3901 AATCGTGCGC AAGGTGCTCA TCGCCAATCG TGGCGAAATC GCTGTCCGCG TGGCCCGGGC
     3961 CTGCCGGGAC GCCGGGATCG CGAGCGTGGC CGTCTACGCG GATCCGGACC GGGACGCGTT
     4021 GCACGTCCGT GCCGCTGATG AGGCGTTCGC CCTGGGTGGT GACACCCCCG CGACCAGCTA
     4081 TCTGGACATC GCCAAGGTCC TCAAAGCCGC GCGCGAGTCG GGCGCGGACG CCATCCACCC
     4141 CGGCTACGGG TTCCTCTCGG AGAACGCCGA GTTCGCGCAG GCGGTCCTGG ACGCCGGCCT
     4201 GATCTGGATC GGCCCGCCCC CGCACGCCAT CCGCGACCTG GGCGACAAGG TCGCCGCCCG
     4261 CCACATCGCC CAGCGGGCCG GCGCCCCCCT GGTCGCCGGC ACCCCCGACC CCGTCTCCGG
     4321 CGCGGACGAG GTCGTCGCCT TCGCCAAGGA GCACGGCCTG CCCATCGCCA TCAAGGCCGC
     4381 CTTCGGCGGC GGCGGGCGCG GCCTCAAGGT CGCCCGCACC CTCGAAGAGG TGCCGGAGCT
     4441 GTACGACTCC GCCGTCCGCG AGGCCGTGGC CGCCTTCGGC CGCGGGGAGT GCTTCGTCGA
     4501 GCGCTACCTC GACAAGCCCC GCCACGTGGA GACCCAGTGC CTGGCCGACA CCCACGGCAA
     4561 CGTGGTCGTC GTCTCCACCC GCGACTGCTC CCTCCAGCGC CGCCACCAAA AGCTCGTCGA
     4621 GGAGGCCCCC GCGCCCTTCC TCTCCGAGGC CCAGACGGAG CAGCTGTACT CATCCTCCAA
     4681 GGCCATCCTG AAGGAGGCCG GCTACGTCGG CGCCGGCACC GTGGAGTTCC TCGTCGGCAT
     4741 GGACGGCACG ATCTCCTTCC TGGAGGTCAA CACCCGCCTC CAGGTCGAGC ACCCGGTCAC
     4801 CGAGGAAGTC GCCGGCATCG ACCTGGTCCG CGAGATGTTC CGCATCGCCG ACGGCGAGGA
     4861 ACTCGGCTAC GACGACCCCG CCCTGCGCGG CCACTCCTTC GAGTTCCGCA TCAACGGCGA
     4921 GGACCCCGGC CGCGGCTTCC TGCCCGCCCC CGGCACCGTC ACCCTCTTCG ACGCGCCCAC
     4981 CGGCCCCGGC GTCCGCCTGG ACGCCGGCGT CGAGTCCGGC TCCGTCATCG GCCCCGCCTG
     5041 GGACTCCCTC CTCGCCAAAC TGATCGTCAC CGGCCGCACC CGCGCCGAGG CACTCCAGCG
     5101 CGCGGCCCGC GCCCTGGACG AGTTCACCGT CGAGGGCATG GCCACCGCCA TCCCCTTCCA
     5161 CCGCACGGTC GTCCGCGACC CGGCCTTCGC CCCCGAACTC ACCGGCTCCA CGGACCCCTT
     5221 CACCGTCCAC ACCCGGTGGA TCGAGACGGA GTTCGTCAAC GAGATCAAGC CCTTCACCAC
     5281 GCCCGCCGAC ACCGAGACGG ACGAGGAGTC GGGCCGGGAG ACGGTCGTCG TCGAGGTCGG
     5341 CGGCAAGCGC CTGGAAGTCT CCCTCCCCTC CAGCCTGGGC ATGTCCCTGG CCCGCACCGG
     5401 CCTGGCCGCC GGGGCCCGCC CCAAGCGCCG CGCGGCCAAG AAGTCCGGCC CCGCCGCCTC
     5461 GGGCGACACC CTCGCCTCCC CGATGCAGGG CACGATCGTC AAGATCGCCG TCGAGGAGGG
     5521 CCAGGAAGTC CAGGAAGGCG ACCTCATCGT CGTACTCGAG GCGATGAAGA TGGAACAGCC
     5581 CCTCAACGCC CACAGGTCCG GCACCATCAA GGGCCTCACC GCCGAGGTCG GCGCCTCCCT
     5641 CACCTCCGGC GCCGCCATCT GCGAGAATCA CTAGCAACTT CATTAAAGAG GAGAAATACT
     5701 AGATGAAGAT TTACGGAATT TATATGGACC GCCCGCTTTC ACAGGAAGAA AATGAACGGT
     5761 TCATGACTTT CATATCACCT GAAAAACGGG AGAAATGCCG GAGATTTTAT CATAAAGAAG
     5821 ATGCTCACCG CACCCTGCTG GGAGATGTGC TCGTTCGCTC AGTCATAAGC AGGCAGTATC
     5881 AGTTGGACAA ATCCGATATC CGCTTTAGCA CGCAGGAATA CGGGAAGCCG TGCATCCCTG
     5941 ATCTTCCCGA CGCTCATTTC AACATTTCTC ACTCCGGCCG CTGGGTCATT GGTGCGTTTG
     6001 ATTCACAGCC GATCGGCATA GATATCGAAA AAACGAAACC GATCAGCCTT GAGATCGCCA
     6061 AGCGCTTCTT TTCAAAAACA GAGTACAGCG ACCTTTTAGC AAAAGACAAG GACGAGCAGA
     6121 CAGACTATTT TTATCATCTA TGGTCAATGA AAGAAAGCTT TATCAAACAG GAAGGCAAAG
     6181 GCTTATCGCT TCCGCTTGAT TCCTTTTCAG TGCGCCTGCA TCAGGACGGA CAAGTATCCA
     6241 TTGAGCTTCC GGACAGCCAT TCCCCATGCT ATATCAAAAC GTATGAGGTC GATCCCGGCT
     6301 ACAAAATGGC TGTATGCGCC GCACACCCTG ATTTCCCCGA GGATATCACA ATGGTCTCGT
     6361 ACGAAGAGCT TTTATAATAC TAGAGCCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA
     6421 GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCTACTA GAGTCACACT
     6481 GGCTCACCTT CGGGTGGGCC TTTCTGCGTT TATATACTAG
//
