LOCUS       BBa_K1150025            7413 bp    DNA     circular UNA 24-OCT-2013
DEFINITION  uniCAS Red Light Stimulator, Freiburg 2013.
ACCESSION   urn.local...1382991827078.31
VERSION     urn.local...1382991827078.31
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..580
                     /note="Geneious type: Promoter"
                     /label="CMV"
     source          <583..595
                     /label="Präfix"
     misc_feature    587..4756
                     /note="Geneious type: dCas9 [RFC 25]"
                     /label="dCas9 [RFC 25]"
     misc_feature    590..595
                     /note="Geneious type: Prefix/Suffix"
                     /label="Prefix"
     misc_feature    596..622
                     /note="Geneious type: Short Element"
                     /label="HA"
     source          623
                     /label="Suffix"
     source          <624..628
                     /label="Präfix"
     misc_feature    629..649
                     /note="Geneious type: Short Element"
                     /label="NLS 1"
     source          650
                     /label="Suffix"
     source          <651..655
                     /label="Präfix"
     misc_feature    3170..3172
                     /note="Geneious type: Mutation"
                     /label="H840A"
     source          4757
                     /label="Suffix"
     misc_feature    4758..4776
                     /note="Geneious type: Short Element"
                     /label="Linker"
     misc_feature    4778..5113
                     /note="Geneious type: Light Part"
                     /label="PIF6 [RFC 25]"
     misc_feature    5084..5104
                     /note="Geneious type: Short Element"
                     /label="NLS 2"
     misc_feature    5105..5110
                     /note="Geneious type: Prefix/Suffix"
                     /label="Suffix"
     source          5105..>5115
                     /label="Suffix"
     terminator      5122..5343
                     /note="Geneious type: Terminator"
                     /label="BgH"
     misc_feature    5345..5364
                     /note="Geneious type: Prefix/Suffix"
                     /label="Suffix"
     source          5364
                     /label="Suffix"
     misc_feature    5604..6218
                     /note="Geneious type: Backbone"
                     /label="Replicon"
     misc_feature    6503..7162
                     /note="Geneious type: Backbone"
                     /label="Chloramphenicol resistance"
     misc_feature    7392..7412
                     /note="Geneious type: Prefix/Suffix"
                     /label="Prefix"
     source          7392
                     /label="Präfix"
ORIGIN      
        1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
       61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagatgg ccggctaccc
      601 atacgatgtt ccagattacg ctaccggccc tgcaagaaaa agattgaata ccggcgacaa
      661 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga
      721 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
      781 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
      841 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
      901 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
      961 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
     1021 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     1081 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     1141 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     1201 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     1261 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     1321 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc
     1381 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     1441 acttcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     1501 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     1561 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     1621 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     1681 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggatacat
     1741 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     1801 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     1861 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     1921 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     1981 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     2041 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     2101 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     2161 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     2221 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     2281 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     2341 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     2401 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     2461 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     2521 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     2581 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     2641 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     2701 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     2761 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     2821 cattgccaat ctggcaggca gccccgccat taagaagggc atcctgcaaa cagtgaaggt
     2881 ggtggacgag ctcgtgaaag tgatgggacg gcacaagccc gagaacatcg tgatcgaaat
     2941 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     3001 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     3061 cacccagctt cagaacgaga agctgtacct gtactacctg caaaatgggc gggatatgta
     3121 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc
     3181 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
     3241 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     3301 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
     3361 cgagagaggc ggcctgagcg aactggataa ggccggwttc atcaagagac agctggtgga
     3421 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     3481 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     3541 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     3601 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     3661 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     3721 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     3781 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     3841 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
     3901 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     3961 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
     4021 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
     4081 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     4141 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     4201 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     4261 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gcgggcgaac tgcaaaaggg
     4321 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     4381 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     4441 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     4501 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     4561 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
     4621 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
     4681 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     4741 tcagctggga ggcgacaccg gcgcaggctc caccggcatg atgttcttac caaccgatta
     4801 ttgttgcagg ttaagcgatc aagagtatat ggagcttgtg tttgagaatg gccagattct
     4861 tgcaaagggc caaagatcca acgtttctct gcataatcaa cgtaccaaat cgatcatgga
     4921 tttgtatgag gcagagtata acgaggattt catgaagagt atcatccatg gtggtggtgg
     4981 tgccatcaca aatctcgggg acacgcaggt tgttccacaa agtcatgttg ctgctgccca
     5041 tgaaacaaac atgttggaaa gcaataaaca tgttgacacc ggccctgcaa gaaaaagatt
     5101 gaataccggt taatactaga ggtgccttct agttgccagc catctgttgt ttgcccctcc
     5161 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5221 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5281 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     5341 atgtactagt agcggccgct gcagtccggc aaaaaagggc aaggtgtcac caccctgccc
     5401 tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc tcactgactc
     5461 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5521 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5581 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga
     5641 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     5701 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     5761 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     5821 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5881 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5941 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6001 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     6061 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6121 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6181 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6241 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6301 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6361 aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc
     6421 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     6481 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta
     6541 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     6601 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     6661 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     6721 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     6781 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     6841 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     6901 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
     6961 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     7021 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     7081 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     7141 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     7201 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     7261 cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     7321 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
     7381 gatgatttct ggaattcgcg gccgcttcta gag
//
