LOCUS       BBa_K1150023            7717 bp    DNA     circular UNA 23-OCT-2013
DEFINITION  uniCAS Histonemodifier, Freiburg 2013.
ACCESSION   urn.local...1382991827043.29
VERSION     urn.local...1382991827043.29
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..344
                     /note="Geneious type: Promoter"
                     /label="SV40"
     source          <347..359
                     /label="Präfix"
     misc_feature    351..4520
                     /note="Geneious type: dCas9 [RFC 25]"
                     /label="dCas9 [RFC 25]"
     misc_feature    354..359
                     /note="Geneious type: Prefix/Suffix"
                     /label="Prefix"
     misc_feature    360..386
                     /note="Geneious type: Short Element"
                     /label="HA"
     source          387
                     /label="Suffix"
     source          <388..392
                     /label="Präfix"
     misc_feature    393..413
                     /note="Geneious type: Short Element"
                     /label="NLS 1"
     source          414
                     /label="Suffix"
     source          <415..419
                     /label="Präfix"
     misc_feature    2934..2936
                     /note="Geneious type: Mutation"
                     /label="H840A"
     source          4521
                     /label="Suffix"
     misc_feature    4522..4540
                     /note="Geneious type: Short Element"
                     /label="Linker"
     misc_feature    4542..5417
                     /note="Geneious type: Effector"
                     /label="G9a [RFC 25]"
     source          <5383..5387
                     /label="Präfix"
     misc_feature    5387^5388
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    5388..5408
                     /note="Geneious type: Short Element"
                     /label="NLS 2"
     misc_feature    5409..5414
                     /note="Geneious type: Prefix/Suffix"
                     /label="Suffix"
     source          5409..>5419
                     /label="Suffix"
     terminator      5426..5647
                     /note="Geneious type: Terminator"
                     /label="BgH"
     misc_feature    5649..5668
                     /note="Geneious type: Prefix/Suffix"
                     /label="Suffix"
     source          5668
                     /label="Suffix"
     misc_feature    5908..6522
                     /note="Geneious type: Backbone"
                     /label="Replicon"
     misc_feature    6807..7466
                     /note="Geneious type: Backbone"
                     /label="Chloramphenicol resistance"
     misc_feature    7696..7716
                     /note="Geneious type: Prefix/Suffix"
                     /label="Prefix"
     source          7696
                     /label="Präfix"
ORIGIN      
        1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
       61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactag atggccggct
      361 acccatacga tgttccagat tacgctaccg gccctgcaag aaaaagattg aataccggcg
      421 acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca
      481 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca
      541 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca
      601 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc
      661 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg
      721 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca
      781 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac
      841 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga
      901 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg
      961 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca
     1021 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc
     1081 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga
     1141 ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg
     1201 ccaaacttca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga
     1261 tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc
     1321 tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga
     1381 tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc
     1441 agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggat
     1501 acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa
     1561 agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc
     1621 agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca
     1681 ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga
     1741 agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat
     1801 tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg
     1861 tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc
     1921 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata
     1981 acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg
     2041 gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga
     2101 agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg
     2161 gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca
     2221 aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga
     2281 ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc
     2341 tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc
     2401 tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt
     2461 tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc
     2521 tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg
     2581 agcacattgc caatctggca ggcagccccg ccattaagaa gggcatcctg caaacagtga
     2641 aggtggtgga cgagctcgtg aaagtgatgg gacggcacaa gcccgagaac atcgtgatcg
     2701 aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga
     2761 agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg
     2821 aaaacaccca gcttcagaac gagaagctgt acctgtacta cctgcaaaat gggcgggata
     2881 tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gacgctatcg
     2941 tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca
     3001 agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact
     3061 actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca
     3121 aggccgagag aggcggcctg agcgaactgg ataaggccgg wttcatcaag agacagctgg
     3181 tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta
     3241 agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc
     3301 tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc
     3361 accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc
     3421 ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga
     3481 tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca
     3541 tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc
     3601 tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca
     3661 ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga
     3721 caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca
     3781 gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt
     3841 ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag
     3901 agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc
     3961 tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact
     4021 ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgcgggc gaactgcaaa
     4081 agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact
     4141 atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc
     4201 acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc
     4261 tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca
     4321 tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg
     4381 ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg
     4441 tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc
     4501 tgtctcagct gggaggcgac accggcgcag gctccaccgg cgagaatgta cccatcccct
     4561 gtgtcaatgg tgtggatggg gagccgtgcc cggaggacta caagtacatc tctgagaact
     4621 gcgagacatc gaccatgaac atcgaccgca acatcaccca tctccagcac tgcacgtgtg
     4681 tggatgactg ctccagctcc aattgcctat gtggtcagct cagtatccga tgctggtatg
     4741 acaaggacgg gcggctgctc caggagttta acaagatcga gccccccctg atctttgagt
     4801 gtaaccaggc atgctcctgc tggagaagct gcaagaaccg cgtggtgcag agcggcatca
     4861 aggtacggct ccagctctac cggactgcca agatgggctg gggggtccga gccttgcaga
     4921 ccatccccca gggcacgttc atctgcgagt atgtaggaga gctgatctct gatgccgagg
     4981 ctgatgtgag agaggatgat tcttacctct tcgatttaga taacaaggat ggcgaggttt
     5041 actgcattga tgcccgttac tatggcaaca tcagccgatt cattaaccac ctgtgtgacc
     5101 ccaacatcat ccctgtccgg gttttcatgc tgcaccaaga tctacggttc ccacgcattg
     5161 ccttcttcag ctccagggac atccggactg gggaggagct gggctttgac tacggtgacc
     5221 gattctggga catcaagagc aagtatttca cctgccagtg tggctctgag aagtgcaagc
     5281 attcagcgga ggccatcgcc ctggagcaga gccgcctggc ccggctggac ccccacccgg
     5341 agctgctccc tgacctcagc tccctgcccc ccatcaacac caccggccct gcaagaaaaa
     5401 gattgaatac cggttaatac tagaggtgcc ttctagttgc cagccatctg ttgtttgccc
     5461 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5521 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5581 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     5641 ctctatgtac tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct
     5701 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
     5761 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5821 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5881 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc
     5941 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6001 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6061 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6121 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6181 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6241 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6301 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6361 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6421 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6481 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6541 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6601 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6661 agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc
     6721 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     6781 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
     6841 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     6901 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     6961 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     7021 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     7081 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     7141 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     7201 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     7261 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     7321 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     7381 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     7441 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     7501 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     7561 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     7621 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     7681 taaggatgat ttctggaatt cgcggccgct tctagag
//
