LOCUS dna 4572 bp CIRCULAR 31-AUG-2013 BASE COUNT 1132 a 1217 c 1157 g 1066 t EMBL_ID dna 4572 bp CIRCULAR 30-AUG-2013 EMBL_ID dna 4572 bp 26-JUL-2013 EMBL_ID dna 4572 bp 06-JUL-2013 EMBL_ID dna 3719 bp EMBL_ID dna 4572 bp 21-MAY-2013 EMBL_ID dna 4572 bp 22-JUL-2013 EMBL_ID dna 4572 bp CIRCULAR 29-AUG-2013 EMBL_ID dna 4572 bp CIRCULAR 30-AUG-2013 UNIMARK dna features dna FEATURES Location/Qualifiers misc_feature 2..29 /gene="lacO reg" /ugene_name="Regulatory_Seq" misc_feature 59..62 /mismatches=0 misc_binding 69..74 /dbxref="REBASE:NcoI" misc_feature 105..108 /mismatches=0 misc_binding 106..111 /dbxref="REBASE:BamHI" misc_binding 112..112 /dbxref="REBASE:EcoRI" misc_feature 121..144 /mismatches=0 /ugene_name="RB25_fw" /ugene_group="Primer" misc_feature 130..864 /note="PPTase (svp)" misc_feature complement(135..153) /mismatches=0 misc_feature 581..584 /mismatches=0 misc_feature 806..809 /mismatches=0 misc_feature 830..833 /mismatches=0 misc_feature complement(843..860) /mismatches=0 misc_feature complement(847..864) /mismatches=0 /ugene_name="RB26_rv" /ugene_group="Primer" misc_feature 850..853 /mismatches=0 misc_feature 883..918 /note="c-myc-tag" misc_feature 905..908 /mismatches=0 misc_feature 995..1158 /5'term="XbaI" /3'term="BglII" /5'overhang="CTAG" /3'overhang="GATC" /5'strand="direct" /3'strand="rev-compl" /5'type="sticky" /3'type="sticky" /fragment_source="dna" /ugene_name="Fragment 2" /ugene_group="fragments" misc_binding 1002..1009 /dbxref="REBASE:NotI" misc_feature 1067..1085 /gene="T7 prom" /ugene_name="Promoter" misc_feature 1085..1112 /gene="lacO reg" /ugene_name="Regulatory_Seq" misc_feature 1140..1143 /mismatches=0 misc_binding 1150..1155 /dbxref="REBASE:NdeI" misc_binding 1158..1163 /dbxref="REBASE:BglII" misc_feature 1163..3157 /5'term="BglII" /3'term="XbaI" /5'overhang="GATC" /3'overhang="CTAG" /5'strand="direct" /3'strand="rev-compl" /5'type="sticky" /3'type="sticky" /fragment_source="dna" /ugene_name="Fragment 3" /ugene_group="fragments" misc_binding 1201..1206 /dbxref="REBASE:KpnI" misc_binding 1207..1212 /dbxref="REBASE:XhoI" misc_feature 1592..2407 /gene="kan2 marker" /ugene_name="Marker" misc_feature 1592..2407 /sequence="ORF_2 rf(6)" /ugene_name="ORF" misc_feature 1640..1643 /mismatches=0 misc_feature 1685..1688 /mismatches=0 misc_feature 1713..1716 /mismatches=0 misc_feature complement(1725..1728) /mismatches=0 misc_feature 1988..1991 /mismatches=0 misc_feature 2003..2006 /mismatches=0 misc_feature 2025..2028 /mismatches=0 misc_feature 2066..2069 /mismatches=0 misc_binding 2099..2104 /dbxref="REBASE:SmaI" misc_binding 2099..2104 /dbxref="REBASE:XmaI" misc_feature 2135..2138 /mismatches=0 misc_binding 2281..2286 /dbxref="REBASE:ClaI" misc_binding 2316..2321 /dbxref="REBASE:NruI" misc_feature complement(2413..2416) /mismatches=0 misc_feature 2449..2477 /gene="amp prom" /ugene_name="Promoter" misc_binding 2503..2508 /dbxref="REBASE:SphI" misc_feature complement(2522..2525) /mismatches=0 misc_feature 2536..2539 /mismatches=0 misc_feature 2539..2542 /mismatches=0 misc_feature 2654..2657 /mismatches=0 misc_feature complement(2705..2708) /mismatches=0 misc_feature complement(2716..2719) /mismatches=0 misc_feature 2977..2980 /mismatches=0 misc_feature 3093..3096 /mismatches=0 misc_feature 3115..3118 /mismatches=0 misc_feature complement(3130..3133) /mismatches=0 misc_binding 3157..3162 /dbxref="REBASE:XbaI" misc_feature join(3162..4572,1..990) /5'term="XbaI" /3'term="XbaI" /5'overhang="CTAG" /3'overhang="CTAG" /5'strand="direct" /3'strand="rev-compl" /5'type="sticky" /3'type="sticky" /fragment_source="dna" /ugene_name="Fragment 1" /ugene_group="fragments" misc_feature 3261..3264 /mismatches=0 misc_feature 3350..4441 /gene="lacI reg" /ugene_name="Regulatory_Seq" misc_feature 3350..4309 /sequence="ORF_1 rf(6)" /ugene_name="ORF" misc_binding 3438..3443 /dbxref="REBASE:NarI" misc_binding 3526..3530 /dbxref="REBASE:AvaII" misc_feature complement(3558..3561) /mismatches=0 misc_binding 3573..3578 /dbxref="REBASE:HpaI" misc_binding 3870..3875 /dbxref="REBASE:ApaI" misc_feature complement(4038..4041) /mismatches=0 misc_binding 4063..4068 /dbxref="REBASE:BclI" misc_feature complement(4540..4543) /mismatches=0 misc_feature 4549..4552 /mismatches=0 ORIGIN 1 GGGGAATTGT GAGCGGATAA CAATTCCCCT GTAGAAATAA TTTTGTTTAA CTTTAATAAG 61 GAGATATACC ATGGGCAGCA GCCATCACCA TCATCACCAC AGCCAGGATC CGAATTCACC 121 ATGACTAGTA TCGCTGCTCT GCTGCCAAGT TGGGCTGTCA CCGAACACGC TTTCACCGAC 181 GCCCCCGACG ACCCTGTCTC CCTGCTGTTT CCCGAAGAGG CAGCTCACGT GGCTCGCGCA 241 GTCCCAAAGC GACTGCATGA GTTCGCAACA GTGAGGGTCT GCGCTAGAGC AGCCCTGGGC 301 AGGCTGGGAC TGCCACCTGG ACCACTGCTG CCTGGGCGGA GGGGTGCCCC ATCATGGCCC 361 GACGGCGTGG TCGGAAGCAT GACTCACTGT CAGGGATTTC GAGGTGCTGC AGTGGCCAGG 421 GCCGCTGACG CAGCCTCTCT GGGAATCGAT GCTGAGCCCA ACGGGCCACT GCCCGATGGT 481 GTGCTGGCCA TGGTCAGCCT GCCTTCCGAG AGAGAATGGC TGGCCGGTCT GGCTGCAAGA 541 CGCCCAGACG TGCATTGGGA TCGCCTGCTG TTCTCTGCCA AGGAGAGTGT CTTTAAGGCC 601 TGGTATCCCC TGACCGGGCT GGAACTGGAC TTCGATGAGG CAGAACTGGC CGTGGACCCT 661 GATGCCGGCA CCTTTACAGC TCGACTGCTG GTGCCTGGAC CAGTGGTCGG CGGACGACGG 721 CTGGACGGAT TCGAGGGACG CTGGGCAGCT GGAGAAGGTC TGGTGGTCAC TGCCATTGCT 781 GTGGCAGCCC CTGCTGGAAC CGCAGAGGAA TCTGCCGAAG GTGCTGGGAA GGAAGCCACT 841 GCCGACGATA GGACTGCCGT GCCAGCTAGC GGTAGCGGAT CCGAACAAAA ACTTATTTCT 901 GAAGAGGATC TGTGATGACG TAACAATTAC GGGTCTACCA TCGAGGGCCT GCTCGATCTC 961 CCGGACGACG ACGCCCCGAA GAGGCGGGGT CTAGAAAGCT TGCGGCCGCA TAATGCTTAA 1021 GTCGAACAGA AAGTAATCGT ATTGTACACG GCCGCATAAT CGAAATTAAT ACGACTCACT 1081 ATAGGGGAAT TGTGAGCGGA TAACAATTCC CCATCTTAGT ATATTAGTTA AGTATAAGAA 1141 GGAGATATAC ATATGGCAGA TCTCAATTGG ATATCGGCCG GCCACGCGAT CGCTGACGTC 1201 GGTACCCTCG AGTCTGGTAA AGAAACCGCT GCTGCGAAAT TTGAACGCCA GCACATGGAC 1261 TCGTCTACTA GCGCAGCTTA ATTAACCTAG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA 1321 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAACC TCAGGCATTT 1381 GAGAAGCACA CGGTCACACT GCTTCCGGTA GTCAATAAAC CGGTAAACCA GCAATAGACA 1441 TAAGCGGCTA TTTAACGACC CTGCCCTGAA CCGACGACAA GCTGACGACC GGGTCTCCGC 1501 AAGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA 1561 TTCAAATATG TATCCGCTCA TGAATTAATT CTTAGAAAAA CTCATCGAGC ATCAAATGAA 1621 ACTGCAATTT ATTCATATCA GGATTATCAA TACCATATTT TTGAAAAAGC CGTTTCTGTA 1681 ATGAAGGAGA AAACTCACCG AGGCAGTTCC ATAGGATGGC AAGATCCTGG TATCGGTCTG 1741 CGATTCCGAC TCGTCCAACA TCAATACAAC CTATTAATTT CCCCTCGTCA AAAATAAGGT 1801 TATCAAGTGA GAAATCACCA TGAGTGACGA CTGAATCCGG TGAGAATGGC AAAAGTTTAT 1861 GCATTTCTTT CCAGACTTGT TCAACAGGCC AGCCATTACG CTCGTCATCA AAATCACTCG 1921 CATCAACCAA ACCGTTATTC ATTCGTGATT GCGCCTGAGC GAGACGAAAT ACGCGGTCGC 1981 TGTTAAAAGG ACAATTACAA ACAGGAATCG AATGCAACCG GCGCAGGAAC ACTGCCAGCG 2041 CATCAACAAT ATTTTCACCT GAATCAGGAT ATTCTTCTAA TACCTGGAAT GCTGTTTTCC 2101 CGGGGATCGC AGTGGTGAGT AACCATGCAT CATCAGGAGT ACGGATAAAA TGCTTGATGG 2161 TCGGAAGAGG CATAAATTCC GTCAGCCAGT TTAGTCTGAC CATCTCATCT GTAACATCAT 2221 TGGCAACGCT ACCTTTGCCA TGTTTCAGAA ACAACTCTGG CGCATCGGGC TTCCCATACA 2281 ATCGATAGAT TGTCGCACCT GATTGCCCGA CATTATCGCG AGCCCATTTA TACCCATATA 2341 AATCAGCATC CATGTTGGAA TTTAATCGCG GCCTAGAGCA AGACGTTTCC CGTTGAATAT 2401 GGCTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 2461 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGCATGCTA GCGCAGAAAC 2521 GTCCTAGAAG ATGCCAGGAG GATACTTAGC AGAGAGACAA TAAGGCCGGA GCGAAGCCGT 2581 TTTTCCATAG GCTCCGCCCC CCTGACGAAC ATCACGAAAT CTGACGCTCA AATCAGTGGT 2641 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGATGGC TCCCTCTTGC 2701 GCTCTCCTGT TCCCGTCCTG CGGCGTCCGT GTTGTGGTGG AGGCTTTACC CAAATCACCA 2761 CGTCCCGTTC CGTGTAGACA GTTCGCTCCA AGCTGGGCTG TGTGCAAGAA CCCCCCGTTC 2821 AGCCCGACTG CTGCGCCTTA TCCGGTAACT ATCATCTTGA GTCCAACCCG GAAAGACACG 2881 ACAAAACGCC ACTGGCAGCA GCCATTGGTA ACTGAGAATT AGTGGATTTA GATATCGAGA 2941 GTCTTGAAGT GGTGGCCTAA CAGAGGCTAC ACTGAAAGGA CAGTATTTGG TATCTGCGCT 3001 CCACTAAAGC CAGTTACCAG GTTAAGCAGT TCCCCAACTG ACTTAACCTT CGATCAAACC 3061 GCCTCCCCAG GCGGTTTTTT CGTTTACAGA GCAGGAGATT ACGACGATCG TAAAAGGATC 3121 TCAAGAAGAT CCTTTACGGA TTCCCGACAC CATCACTCTA GATTTCAGTG CAATTTATCT 3181 CTTCAAATGT AGCACCTGAA GTCAGCCCCA TACGATATAA GTTGTAATTC TCATGTTAGT 3241 CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG 3301 AGATCCCGGT GCCTAATGAG TGAGCTAACT TACATTAATT GCGTTGCGCT CACTGCCCGC 3361 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG 3421 AGGCGGTTTG CGTATTGGGC GCCAGGGTGG TTTTTCTTTT CACCAGTGAG ACGGGCAACA 3481 GCTGATTGCC CTTCACCGCC TGGCCCTGAG AGAGTTGCAG CAAGCGGTCC ACGCTGGTTT 3541 GCCCCAGCAG GCGAAAATCC TGTTTGATGG TGGTTAACGG CGGGATATAA CATGAGCTGT 3601 CTTCGGTATC GTCGTATCCC ACTACCGAGA TGTCCGCACC AACGCGCAGC CCGGACTCGG 3661 TAATGGCGCG CATTGCGCCC AGCGCCATCT GATCGTTGGC AACCAGCATC GCAGTGGGAA 3721 CGATGCCCTC ATTCAGCATT TGCATGGTTT GTTGAAAACC GGACATGGCA CTCCAGTCGC 3781 CTTCCCGTTC CGCTATCGGC TGAATTTGAT TGCGAGTGAG ATATTTATGC CAGCCAGCCA 3841 GACGCAGACG CGCCGAGACA GAACTTAATG GGCCCGCTAA CAGCGCGATT TGCTGGTGAC 3901 CCAATGCGAC CAGATGCTCC ACGCCCAGTC GCGTACCGTC TTCATGGGAG AAAATAATAC 3961 TGTTGATGGG TGTCTGGTCA GAGACATCAA GAAATAACGC CGGAACATTA GTGCAGGCAG 4021 CTTCCACAGC AATGGCATCC TGGTCATCCA GCGGATAGTT AATGATCAGC CCACTGACGC 4081 GTTGCGCGAG AAGATTGTGC ACCGCCGCTT TACAGGCTTC GACGCCGCTT CGTTCTACCA 4141 TCGACACCAC CACGCTGGCA CCCAGTTGAT CGGCGCGAGA TTTAATCGCC GCGACAATTT 4201 GCGACGGCGC GTGCAGGGCC AGACTGGAGG TGGCAACGCC AATCAGCAAC GACTGTTTGC 4261 CCGCCAGTTG TTGTGCCACG CGGTTGGGAA TGTAATTCAG CTCCGCCATC GCCGCTTCCA 4321 CTTTTTCCCG CGTTTTCGCA GAAACGTGGC TGGCCTGGTT CACCACGCGG GAAACGGTCT 4381 GATAAGAGAC ACCGGCATAC TCTGCGACAT CGTATAACGT TACTGGTTTC ACATTCACCA 4441 CCCTGAATTG ACTCTCTTCC GGGCGCTATC ATGCCATACC GCGAAAGGTT TTGCGCCATT 4501 CGATGGTGTC CGGGATCTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAATTAATA 4561 CGACTCACTA TA //