LOCUS pKH6-BBa_sfp_Bsub_CDS 2746 bp 11-AUG-2013 EMBL_ID pKH6-BBa_sfp_Bsub_CDS 2746 bp 09-AUG-2013 UNIMARK pKH6-BBa_sfp_Bsub_CDS annotations pKH6-BBa_sfp_Bsub_CDS FEATURES Location/Qualifiers source 1..2746 /comment="Molecule is created with Unipro UGENE v1.12" stem_loop 2..73 /label="E. coli his operon terminator" stem_loop 10..44 /label="Stem loop" primer_bind complement(137..156) /label="Verification reverse (VR) primer binding site" misc_feature complement(241..855) /gene="rep (pMB1)" /ugene_name="misc" misc_feature 256..256 /gene="ORI" /ugene_name="misc" stem_loop 1022..1127 /label="T0 terminator" CDS complement(1140..1799) /gene="Chloramphenicol resistance" primer_bind 1913..1932 /label="Verification forward (VF2) primer binding site" stem_loop complement(1989..1992) /label="end of terminator" stem_loop complement(2002..2021) /label="Stem loop" misc_feature 2029..2075 /mismatches=0 /ugene_name="KH15_fw" /ugene_group="Primer" misc_feature 2049..2726 /mismatches=0 /ugene_name="sfp_Bsub" /ugene_group="gene" misc_feature complement(join(2700..2746,1..1)) /mismatches=0 /ugene_name="KH16_rv" /ugene_group="Primer" ORIGIN 1 GTCCGGCAAA AAAGGGCAAG GTGTCACCAC CCTGCCCTTT TTCTTTAAAA CCGAAAAGAT 61 TACTTCGCGT TATGCAGGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 121 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 181 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 241 CGTTGCTGGC GTTTTTCCAC AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 301 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 361 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 421 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 481 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 541 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 601 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 661 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 721 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 781 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 841 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 901 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 961 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGCTCGAGGC 1021 TTGGATTCTC ACCAATAAAA AACGCCCGGC GGCAACCGAG CGTTCTGAAC AAATCCAGAT 1081 GGAGTTCTGA GGTCATTACT GGATCTATCA ACAGGAGTCC AAGCGAGCTC GATATCAAAT 1141 TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA 1201 TGGAAGCCAT CACAAACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG 1261 CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC 1321 ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC 1381 TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA 1441 TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT 1501 TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA 1561 CCGTCTTTCA TTGCCATACG AAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA 1621 ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA 1681 TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT 1741 TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT 1801 TTAGCTTCCT TAGCTCCTGA AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT 1861 ATTTCATTAT GGTGAAAGTT GGAACCTCTT ACGTGCCCGA TCAACTCGAG TGCCACCTGA 1921 CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCA 1981 GAATTTCAGA TAAAAAAAAT CCTTAGCTTT CGCTAAGGAT GATTTCTGGA ATTCGCGGCC 2041 GCTTCTAGAT GAAGATTTAC GGAATTTATA TGGACCGCCC GCTTTCACAG GAAGAAAATG 2101 AACGGTTCAT GACTTTCATA TCACCTGAAA AACGGGAGAA ATGCCGGAGA TTTTATCATA 2161 AAGAAGATGC TCACCGCACC CTGCTGGGAG ATGTGCTCGT TCGCTCAGTC ATAAGCAGGC 2221 AGTATCAGTT GGACAAATCC GATATCCGCT TTAGCACGCA GGAATACGGG AAGCCGTGCA 2281 TCCCTGATCT TCCCGACGCT CATTTCAACA TTTCTCACTC CGGCCGCTGG GTCATTGGTG 2341 CGTTTGATTC ACAGCCGATC GGCATAGATA TCGAAAAAAC GAAACCGATC AGCCTTGAGA 2401 TCGCCAAGCG CTTCTTTTCA AAAACAGAGT ACAGCGACCT TTTAGCAAAA GACAAGGACG 2461 AGCAGACAGA CTATTTTTAT CATCTATGGT CAATGAAAGA AAGCTTTATC AAACAGGAAG 2521 GCAAAGGCTT ATCGCTTCCG CTTGATTCCT TTTCAGTGCG CCTGCATCAG GACGGACAAG 2581 TATCCATTGA GCTTCCGGAC AGCCATTCCC CATGCTATAT CAAAACGTAT GAGGTCGATC 2641 CCGGCTACAA AATGGCTGTA TGCGCCGCAC ACCCTGATTT CCCCGAGGAT ATCACAATGG 2701 TCTCGTACGA AGAGCTTTTA TAATGATACT AGTAGCGGCC GCTGCA //