LOCUS       BBa_K1150027            5268 bp    DNA     circular UNA 24-OCT-2013
DEFINITION  uniCAS Red Light Repressor, Freiburg 2013.
ACCESSION   urn.local...1382991827112.33
VERSION     urn.local...1382991827112.33
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..580
                     /note="Geneious type: Promoter"
                     /label="CMV"
     source          <583..>586
                     /label="Präfix"
     misc_feature    587..2545
                     /note="Geneious type: Light Part"
                     /label="PhyB [RFC 25]"
     misc_feature    590..595
                     /note="Geneious type: Prefix/Suffix"
                     /label="Prefix"
     misc_feature    2546..2566
                     /note="Geneious type: Short Element"
                     /label="Linker"
     misc_feature    2567..2968
                     /note="Geneious type: Effector"
                     /label="KRAB [RFC 25]"
     misc_feature    2939..2959
                     /note="Geneious type: Short Element"
                     /label="NLS"
     misc_feature    2960..2965
                     /note="Geneious type: Prefix/Suffix"
                     /label="Suffix"
     terminator      2977..3198
                     /note="Geneious type: Terminator"
                     /label="BgH"
     misc_feature    3200..3219
                     /note="Geneious type: Prefix/Suffix"
                     /label="Suffix"
     source          3219
                     /label="Suffix"
     misc_feature    3459..4073
                     /note="Geneious type: Backbone"
                     /label="Replicon"
     misc_feature    4358..5017
                     /note="Geneious type: Backbone"
                     /label="Chloramphenicol resistance"
     source          5247
                     /label="Präfix"
     misc_feature    5247..5267
                     /note="Geneious type: Prefix/Suffix"
                     /label="Prefix"
ORIGIN      
        1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
       61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagatgg ccggcatggt
      601 ttccggagtc gggggtagtg gcggtggccg tggcggtggc cgtggcggag aagaagaacc
      661 gtcgtcaagt cacactccta ataaccgaag aggaggagaa caagctcaat cgtcgggaac
      721 gaaatctctc agaccaagaa gcaacactga atcaatgagc aaagcaattc aacagtacac
      781 cgtcgacgca agactccacg ccgttttcga acaatccggc gaatcaggga aatcattcga
      841 ctactcacaa tcactcaaaa cgacgacgta cggttcctct gtacctgagc aacagatcac
      901 agcttatctc tctcgaatcc agcgaggtgg ttacattcag cctttcggat gtatgatcgc
      961 cgtcgatgaa tccagtttcc ggatcatcgg ttacagtgaa aacgccagag aaatgttagg
     1021 gattatgcct caatctgttc ctactcttga gaaacctgag attctagcta tgggaactga
     1081 tgtgagatct ttgttcactt cttcgagctc gattctactc gagcgtgctt tcgttgctcg
     1141 agagattacc ttgttaaatc cggtttggat ccattccaag aatactggta aaccgtttta
     1201 cgccattctt cataggattg atgttggtgt tgttattgat ttagagccag ctagaactga
     1261 agatcctgcg ctttctattg ctggtgctgt tcaatcgcag aaactcgcgg ttcgtgcgat
     1321 ttctcagtta caggctcttc ctggtggaga tattaagctt ttgtgtgaca ctgtcgtgga
     1381 aagtgtgagg gacttgactg gttatgatcg tgttatggtt tataagtttc atgaagatga
     1441 gcatggagaa gttgtagctg agagtaaacg agatgattta gagccttata ttggactgca
     1501 ttatcctgct actgatattc ctcaagcgtc aaggttcttg tttaagcaga accgtgtccg
     1561 aatgatagta gattgcaatg ccacacctgt tcttgtggtc caggacgata ggctaactca
     1621 gtctatgtgc ttggttggtt ctactcttag ggctcctcat ggttgtcact ctcagtatat
     1681 ggctaacatg ggatctattg cgtctttagc aatggcggtt ataatcaatg gaaatgaaga
     1741 tgatgggagc aatgtagcta gtggaagaag ctcgatgagg ctttggggtt tggttgtttg
     1801 ccatcacact tcttctcgct gcataccgtt tccgctaagg tatgcttgtg agtttttgat
     1861 gcaggctttc ggtttacagt taaacatgga attgcagtta gctttgcaaa tgtcagagaa
     1921 acgcgttttg agaacgcaga cactgttatg tgatatgctt ctgcgtgact cgcctgctgg
     1981 aattgttaca cagagtccca gtatcatgga cttagtgaaa tgtgacggtg cagcatttct
     2041 ttaccacggg aagtattacc cgttgggtgt tgctcctagt gaagttcaga taaaagatgt
     2101 tgtggagtgg ttgcttgcga atcatgcgga ttcaaccgga ttaagcactg atagtttagg
     2161 cgatgcgggg tatcccggtg cagctgcgtt aggggatgct gtgtgcggta tggcagttgc
     2221 atatatcaca aaaagagact ttcttttttg gtttcgatct cacactgcga aagaaatcaa
     2281 atggggaggc gctaagcatc atccggagga taaagatgat gggcaacgaa tgcatcctcg
     2341 ttcgtccttt caggcttttc ttgaagttgt taagagccgg agtcagccat gggaaactgc
     2401 ggaaatggat gcgattcact cgctccagct tattctgaga gactctttta aagaatctga
     2461 ggcggctatg aactctaaag ttgtggatgg tgtggttcag ccatgtaggg atatggcggg
     2521 ggaacagggg attgatgagt taggtaccgg cgcaggctcc accggcatgg atgctaagtc
     2581 actaactgcc tggtcccgga cactggtgac cttcaaggat gtatttgtgg acttcaccag
     2641 ggaggagtgg aagctgctgg acactgctca gcagatcgtg tacagaaatg tgatgctgga
     2701 gaactataag aacctggttt ccttgggtta tcagcttact aagccagatg tgatcctccg
     2761 gttggagaag ggagaagagc cctggctggt ggagagagaa attcaccaag agacccatcc
     2821 tgattcagag actgcatttg aaatcaaatc atcagtttcc agcaggagca tttttaaaga
     2881 taagcaatcc tgtgacatta aaatggaagg aatggcaagg aatgatctct ggaccggccc
     2941 tgcaagaaaa agattgaata ccggttaata ctagaggtgc cttctagttg ccagccatct
     3001 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3061 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3121 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3181 gatgcggtgg gctctatgta ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt
     3241 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc
     3301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag
     3481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3901 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4201 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa
     4261 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     4321 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     4381 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     4441 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     4501 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     4561 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     4621 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     4681 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     4741 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     4801 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     4861 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     4921 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     4981 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     5041 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     5101 aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc
     5161 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc
     5221 ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagag
//
