LOCUS BBa_K1150022 7479 bp DNA circular UNA 23-OCT-2013 DEFINITION uniCAS Repressor, Freiburg 2013. ACCESSION urn.local...1382991827016.28 VERSION urn.local...1382991827016.28 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..580 /note="Geneious type: Promoter" /label="CMV" source <583..595 /label="Präfix" misc_feature 587..4756 /note="Geneious type: dCas9 [RFC 25]" /label="dCas9 [RFC 25]" misc_feature 590..595 /note="Geneious type: Prefix/Suffix" /label="Prefix" misc_feature 596..622 /note="Geneious type: Short Element" /label="HA" source 623 /label="Suffix" source <624..628 /label="Präfix" misc_feature 629..649 /note="Geneious type: Short Element" /label="NLS 1" source 650 /label="Suffix" source <651..655 /label="Präfix" misc_feature 3170..3172 /note="Geneious type: Mutation" /label="H840A" source 4757 /label="Suffix" misc_feature 4758..4776 /note="Geneious type: Short Element" /label="Linker" misc_feature 4778..5179 /note="Geneious type: Effector" /label="KRAB [RFC 25]" source <5145..5149 /label="Präfix" misc_feature 5149^5150 /note="Geneious type: origin" /label="origin" misc_feature 5150..5170 /note="Geneious type: Short Element" /label="NLS 2" misc_feature 5171..5176 /note="Geneious type: Prefix/Suffix" /label="Suffix" source 5171..>5181 /label="Suffix" terminator 5188..5409 /note="Geneious type: Terminator" /label="BgH" misc_feature 5411..5430 /note="Geneious type: Prefix/Suffix" /label="Suffix" source 5430 /label="Suffix" misc_feature 5670..6284 /note="Geneious type: Backbone" /label="Replicon" misc_feature 6569..7228 /note="Geneious type: Backbone" /label="Chloramphenicol resistance" misc_feature 7458..7478 /note="Geneious type: Prefix/Suffix" /label="Prefix" source 7458 /label="Präfix" ORIGIN 1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagatgg ccggctaccc 601 atacgatgtt ccagattacg ctaccggccc tgcaagaaaa agattgaata ccggcgacaa 661 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga 721 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat 781 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg 841 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca 901 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga 961 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt 1021 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt 1081 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa 1141 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa 1201 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc 1261 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga 1321 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc 1381 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa 1441 acttcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg 1501 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag 1561 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa 1621 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct 1681 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggatacat 1741 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat 1801 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg 1861 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct 1921 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat 1981 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc 2041 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga 2101 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc 2161 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga 2221 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga 2281 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca 2341 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt 2401 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga 2461 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct 2521 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt 2581 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag 2641 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct 2701 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac 2761 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca 2821 cattgccaat ctggcaggca gccccgccat taagaagggc atcctgcaaa cagtgaaggt 2881 ggtggacgag ctcgtgaaag tgatgggacg gcacaagccc gagaacatcg tgatcgaaat 2941 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg 3001 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa 3061 cacccagctt cagaacgaga agctgtacct gtactacctg caaaatgggc gggatatgta 3121 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc 3181 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa 3241 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg 3301 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc 3361 cgagagaggc ggcctgagcg aactggataa ggccggwttc atcaagagac agctggtgga 3421 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta 3481 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt 3541 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca 3601 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa 3661 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc 3721 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat 3781 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat 3841 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt 3901 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg 3961 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa 4021 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt 4081 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct 4141 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga 4201 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct 4261 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gcgggcgaac tgcaaaaggg 4321 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga 4381 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa 4441 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc 4501 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag 4561 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc 4621 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct 4681 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc 4741 tcagctggga ggcgacaccg gcgcaggctc caccggcatg gatgctaagt cactaactgc 4801 ctggtcccgg acactggtga ccttcaagga tgtatttgtg gacttcacca gggaggagtg 4861 gaagctgctg gacactgctc agcagatcgt gtacagaaat gtgatgctgg agaactataa 4921 gaacctggtt tccttgggtt atcagcttac taagccagat gtgatcctcc ggttggagaa 4981 gggagaagag ccctggctgg tggagagaga aattcaccaa gagacccatc ctgattcaga 5041 gactgcattt gaaatcaaat catcagtttc cagcaggagc atttttaaag ataagcaatc 5101 ctgtgacatt aaaatggaag gaatggcaag gaatgatctc tggaccggcc ctgcaagaaa 5161 aagattgaat accggttaat actagaggtg ccttctagtt gccagccatc tgttgtttgc 5221 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 5281 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 5341 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 5401 ggctctatgt actagtagcg gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc 5461 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac 5521 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 5581 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 5641 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc 5701 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 5761 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 5821 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 5881 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 5941 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 6001 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 6061 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 6121 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 6181 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 6241 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 6301 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 6361 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 6421 tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg 6481 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa 6541 caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact 6601 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct 6661 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa 6721 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc 6781 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt 6841 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt 6901 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag 6961 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga aattccggat 7021 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt 7081 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt 7141 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg 7201 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata 7261 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta 7321 cgtgcccgat caactcgagt gccacctgac gtctaagaaa ccattattat catgacatta 7381 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc 7441 gctaaggatg atttctggaa ttcgcggccg cttctagag //