LOCUS       BBa_K1150018            6856 bp    DNA     circular UNA 24-OCT-2013
DEFINITION  uniCAS, Freiburg 2013.
ACCESSION   urn.local...1382991826894.24
VERSION     urn.local...1382991826894.24
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..344
                     /note="Geneious type: Promoter"
                     /label="SV40"
     source          <347..359
                     /label="Präfix"
     misc_feature    351..4556
                     /note="Geneious type: dCas9 [RFC 25]"
                     /label="dCas9 [RFC 25]"
     misc_feature    354..359
                     /note="Geneious type: Prefix/Suffix"
                     /label="Prefix"
     misc_feature    360..386
                     /note="Geneious type: Short Element"
                     /label="HA"
     source          387
                     /label="Suffix"
     source          <388..392
                     /label="Präfix"
     misc_feature    393..413
                     /note="Geneious type: Short Element"
                     /label="NLS 1"
     source          414
                     /label="Suffix"
     source          <415..419
                     /label="Präfix"
     misc_feature    2934..2936
                     /note="Geneious type: Mutation"
                     /label="H840A"
     misc_feature    4527..4547
                     /note="Geneious type: Short Element"
                     /label="NLS 2"
     terminator      4565..4786
                     /note="Geneious type: Terminator"
                     /label="BgH"
     misc_feature    4788..4807
                     /note="Geneious type: Prefix/Suffix"
                     /label="Suffix"
     source          4807
                     /label="Suffix"
     misc_feature    5047..5661
                     /note="Geneious type: Backbone"
                     /label="Replicon"
     misc_feature    5946..6605
                     /note="Geneious type: Backbone"
                     /label="Chloramphenicol resistance"
     misc_feature    6835..6855
                     /note="Geneious type: Prefix/Suffix"
                     /label="Prefix"
     source          6835
                     /label="Präfix"
ORIGIN      
        1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
       61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactag atggccggct
      361 acccatacga tgttccagat tacgctaccg gccctgcaag aaaaagattg aataccggcg
      421 acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca
      481 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca
      541 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca
      601 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc
      661 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg
      721 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca
      781 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac
      841 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga
      901 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg
      961 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca
     1021 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc
     1081 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga
     1141 ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg
     1201 ccaaacttca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga
     1261 tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc
     1321 tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga
     1381 tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc
     1441 agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggat
     1501 acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa
     1561 agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc
     1621 agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca
     1681 ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga
     1741 agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat
     1801 tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg
     1861 tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc
     1921 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata
     1981 acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg
     2041 gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga
     2101 agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg
     2161 gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca
     2221 aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga
     2281 ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc
     2341 tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc
     2401 tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt
     2461 tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc
     2521 tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg
     2581 agcacattgc caatctggca ggcagccccg ccattaagaa gggcatcctg caaacagtga
     2641 aggtggtgga cgagctcgtg aaagtgatgg gacggcacaa gcccgagaac atcgtgatcg
     2701 aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga
     2761 agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg
     2821 aaaacaccca gcttcagaac gagaagctgt acctgtacta cctgcaaaat gggcgggata
     2881 tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gacgctatcg
     2941 tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca
     3001 agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact
     3061 actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca
     3121 aggccgagag aggcggcctg agcgaactgg ataaggccgg wttcatcaag agacagctgg
     3181 tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta
     3241 agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc
     3301 tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc
     3361 accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc
     3421 ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga
     3481 tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca
     3541 tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc
     3601 tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca
     3661 ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga
     3721 caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca
     3781 gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt
     3841 ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag
     3901 agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc
     3961 tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact
     4021 ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgcgggc gaactgcaaa
     4081 agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact
     4141 atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc
     4201 acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc
     4261 tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca
     4321 tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg
     4381 ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg
     4441 tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc
     4501 tgtctcagct gggaggcgac accggccctg caagaaaaag attgaatacc ggttaatact
     4561 agaggtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4621 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4681 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     4741 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatgtact agtagcggcc
     4801 gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
     4861 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4921 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4981 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5041 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
     5101 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5161 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5221 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5281 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5341 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5401 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5461 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5521 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5581 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5641 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5701 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5761 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
     5821 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     5881 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     5941 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     6001 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     6061 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     6121 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     6181 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     6241 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     6301 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     6361 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     6421 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     6481 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     6541 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     6601 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     6661 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc
     6721 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     6781 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
     6841 gcggccgctt ctagag
//
