LOCUS BBa_K1150024 7953 bp DNA circular UNA 24-OCT-2013 DEFINITION uniCAS Histonemodifier, Freiburg 2013. ACCESSION urn.local...1382991827062.30 VERSION urn.local...1382991827062.30 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..580 /note="Geneious type: Promoter" /label="CMV" source <583..595 /label="Präfix" misc_feature 587..4756 /note="Geneious type: dCas9 [RFC 25]" /label="dCas9 [RFC 25]" misc_feature 590..595 /note="Geneious type: Prefix/Suffix" /label="Prefix" misc_feature 596..622 /note="Geneious type: Short Element" /label="HA" source 623 /label="Suffix" source <624..628 /label="Präfix" misc_feature 629..649 /note="Geneious type: Short Element" /label="NLS 1" source 650 /label="Suffix" source <651..655 /label="Präfix" misc_feature 3170..3172 /note="Geneious type: Mutation" /label="H840A" misc_feature 4757..4777 /note="Geneious type: Short Element" /label="Linker" source 4757 /label="Suffix" misc_feature 4778..5653 /note="Geneious type: Effector" /label="G9a [RFC 25]" source <5619..5623 /label="Präfix" misc_feature 5623^5624 /note="Geneious type: origin" /label="origin" misc_feature 5624..5644 /note="Geneious type: Short Element" /label="NLS 2" misc_feature 5645..5650 /note="Geneious type: Prefix/Suffix" /label="Suffix" source 5645..>5655 /label="Suffix" terminator 5662..5883 /note="Geneious type: Terminator" /label="BgH" misc_feature 5885..5904 /note="Geneious type: Prefix/Suffix" /label="Suffix" source 5904 /label="Suffix" misc_feature 6144..6758 /note="Geneious type: Backbone" /label="Replicon" misc_feature 7043..7702 /note="Geneious type: Backbone" /label="Chloramphenicol resistance" misc_feature 7932..7952 /note="Geneious type: Prefix/Suffix" /label="Prefix" source 7932 /label="Präfix" ORIGIN 1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagatgg ccggctaccc 601 atacgatgtt ccagattacg ctaccggccc tgcaagaaaa agattgaata ccggcgacaa 661 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga 721 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat 781 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg 841 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca 901 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga 961 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt 1021 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt 1081 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa 1141 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa 1201 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc 1261 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga 1321 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc 1381 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa 1441 acttcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg 1501 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag 1561 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa 1621 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct 1681 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggatacat 1741 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat 1801 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg 1861 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct 1921 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat 1981 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc 2041 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga 2101 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc 2161 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga 2221 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga 2281 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca 2341 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt 2401 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga 2461 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct 2521 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt 2581 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag 2641 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct 2701 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac 2761 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca 2821 cattgccaat ctggcaggca gccccgccat taagaagggc atcctgcaaa cagtgaaggt 2881 ggtggacgag ctcgtgaaag tgatgggacg gcacaagccc gagaacatcg tgatcgaaat 2941 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg 3001 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa 3061 cacccagctt cagaacgaga agctgtacct gtactacctg caaaatgggc gggatatgta 3121 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc 3181 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa 3241 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg 3301 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc 3361 cgagagaggc ggcctgagcg aactggataa ggccggwttc atcaagagac agctggtgga 3421 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta 3481 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt 3541 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca 3601 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa 3661 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc 3721 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat 3781 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat 3841 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt 3901 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg 3961 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa 4021 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt 4081 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct 4141 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga 4201 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct 4261 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gcgggcgaac tgcaaaaggg 4321 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga 4381 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa 4441 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc 4501 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag 4561 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc 4621 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct 4681 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc 4741 tcagctggga ggcgacaccg gcgcaggctc caccggcgag aatgtaccca tcccctgtgt 4801 caatggtgtg gatggggagc cgtgcccgga ggactacaag tacatctctg agaactgcga 4861 gacatcgacc atgaacatcg accgcaacat cacccatctc cagcactgca cgtgtgtgga 4921 tgactgctcc agctccaatt gcctatgtgg tcagctcagt atccgatgct ggtatgacaa 4981 ggacgggcgg ctgctccagg agtttaacaa gatcgagccc cccctgatct ttgagtgtaa 5041 ccaggcatgc tcctgctgga gaagctgcaa gaaccgcgtg gtgcagagcg gcatcaaggt 5101 acggctccag ctctaccgga ctgccaagat gggctggggg gtccgagcct tgcagaccat 5161 cccccagggc acgttcatct gcgagtatgt aggagagctg atctctgatg ccgaggctga 5221 tgtgagagag gatgattctt acctcttcga tttagataac aaggatggcg aggtttactg 5281 cattgatgcc cgttactatg gcaacatcag ccgattcatt aaccacctgt gtgaccccaa 5341 catcatccct gtccgggttt tcatgctgca ccaagatcta cggttcccac gcattgcctt 5401 cttcagctcc agggacatcc ggactgggga ggagctgggc tttgactacg gtgaccgatt 5461 ctgggacatc aagagcaagt atttcacctg ccagtgtggc tctgagaagt gcaagcattc 5521 agcggaggcc atcgccctgg agcagagccg cctggcccgg ctggaccccc acccggagct 5581 gctccctgac ctcagctccc tgccccccat caacaccacc ggccctgcaa gaaaaagatt 5641 gaataccggt taatactaga ggtgccttct agttgccagc catctgttgt ttgcccctcc 5701 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 5761 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 5821 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct 5881 atgtactagt agcggccgct gcagtccggc aaaaaagggc aaggtgtcac caccctgccc 5941 tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc tcactgactc 6001 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 6061 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 6121 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga 6181 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 6241 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 6301 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 6361 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 6421 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 6481 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 6541 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 6601 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 6661 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 6721 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 6781 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 6841 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 6901 aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc 6961 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag 7021 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta 7081 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg 7141 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg 7201 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat 7261 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac 7321 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt 7381 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa 7441 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat 7501 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta 7561 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa 7621 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat 7681 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa 7741 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc 7801 cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 7861 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag 7921 gatgatttct ggaattcgcg gccgcttcta gag //