LOCUS BBa_K1150019 7249 bp DNA circular UNA 23-OCT-2013 DEFINITION uniCAS Activator, Freiburg 2013. ACCESSION urn.local...1382991826925.25 VERSION urn.local...1382991826925.25 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..344 /note="Geneious type: Promoter" /label="SV40" source <347..359 /label="Präfix" misc_feature 351..4520 /note="Geneious type: dCas9 [RFC 25]" /label="dCas9 [RFC 25]" misc_feature 354..359 /note="Geneious type: Prefix/Suffix" /label="Prefix" misc_feature 360..386 /note="Geneious type: Short Element" /label="HA" source 387 /label="Suffix" source <388..392 /label="Präfix" misc_feature 393..413 /note="Geneious type: Short Element" /label="NLS 1" source 414 /label="Suffix" source <415..419 /label="Präfix" misc_feature 2934..2936 /note="Geneious type: Mutation" /label="H840A" misc_feature 4521..4541 /note="Geneious type: Short Element" /label="Linker" source 4521 /label="Suffix" misc_feature 4542..4949 /note="Geneious type: Effector" /label="VP16 [RFC 25]" source <4915..4919 /label="Präfix" misc_feature 4919^4920 /note="Geneious type: origin" /label="origin" misc_feature 4920..4940 /note="Geneious type: Short Element" /label="NLS 2" misc_feature 4941..4946 /note="Geneious type: Prefix/Suffix" /label="Suffix" source 4941..>4951 /label="Suffix" terminator 4958..5179 /note="Geneious type: Terminator" /label="BgH" misc_feature 5181..5200 /note="Geneious type: Prefix/Suffix" /label="Suffix" source 5200 /label="Suffix" misc_feature 5440..6054 /note="Geneious type: Backbone" /label="Replicon" misc_feature 6339..6998 /note="Geneious type: Backbone" /label="Chloramphenicol resistance" misc_feature 7228..7248 /note="Geneious type: Prefix/Suffix" /label="Prefix" source 7228 /label="Präfix" ORIGIN 1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactag atggccggct 361 acccatacga tgttccagat tacgctaccg gccctgcaag aaaaagattg aataccggcg 421 acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca 481 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca 541 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca 601 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc 661 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg 721 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca 781 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac 841 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga 901 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg 961 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca 1021 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc 1081 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga 1141 ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg 1201 ccaaacttca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga 1261 tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc 1321 tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga 1381 tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc 1441 agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggat 1501 acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa 1561 agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc 1621 agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca 1681 ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga 1741 agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat 1801 tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg 1861 tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc 1921 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata 1981 acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg 2041 gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga 2101 agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg 2161 gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca 2221 aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga 2281 ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc 2341 tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc 2401 tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt 2461 tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc 2521 tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg 2581 agcacattgc caatctggca ggcagccccg ccattaagaa gggcatcctg caaacagtga 2641 aggtggtgga cgagctcgtg aaagtgatgg gacggcacaa gcccgagaac atcgtgatcg 2701 aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga 2761 agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg 2821 aaaacaccca gcttcagaac gagaagctgt acctgtacta cctgcaaaat gggcgggata 2881 tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gacgctatcg 2941 tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca 3001 agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact 3061 actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca 3121 aggccgagag aggcggcctg agcgaactgg ataaggccgg wttcatcaag agacagctgg 3181 tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta 3241 agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc 3301 tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc 3361 accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc 3421 ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga 3481 tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca 3541 tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc 3601 tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca 3661 ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga 3721 caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca 3781 gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt 3841 ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag 3901 agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc 3961 tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact 4021 ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgcgggc gaactgcaaa 4081 agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact 4141 atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc 4201 acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc 4261 tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca 4321 tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg 4381 ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg 4441 tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc 4501 tgtctcagct gggaggcgac accggcgcag gctccaccgg cgcgcgtacg aaaaacaatt 4561 acgggtctac catcgagggc ctgctcgatc tcccggacga cgacgccccc gaagaggcgg 4621 ggctggcggc tccgcgcctg tcctttctcc ccgcgggaca cacgcgcaga ctgtcgacgg 4681 cccccccgac cgatgtcagc ctgggggacg agctccactt agacggcgag gacgtggcga 4741 tggcgcatgc cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc 4801 cgggtccggg atttaccccc cacgactccg ccccctacgg cgctctggat atggccgact 4861 tcgagtttga gcagatgttt accgatgccc ttggaattga cgagtacggt gggaccggcc 4921 ctgcaagaaa aagattgaat accggttaat actagaggtg ccttctagtt gccagccatc 4981 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 5041 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 5101 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 5161 ggatgcggtg ggctctatgt actagtagcg gccgctgcag tccggcaaaa aagggcaagg 5221 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt 5281 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 5341 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 5401 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca 5461 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 5521 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 5581 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 5641 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 5701 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 5761 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 5821 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 5881 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 5941 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 6001 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 6061 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 6121 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 6181 aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa 6241 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg 6301 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca 6361 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca 6421 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 6481 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg 6541 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa 6601 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg 6661 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg 6721 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga 6781 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg 6841 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta 6901 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat 6961 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa 7021 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg 7081 gaacctctta cgtgcccgat caactcgagt gccacctgac gtctaagaaa ccattattat 7141 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc 7201 cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagag //