Team:Kyoto/Notebook

{{Kyoto/header}}
<div id="kyoto-main">
<div class="texts">
<div id="notebooks">

{{Kyoto/Notebook/Calender}}
==template==
===Transformation===
<!-- こっから -->
<div class="experiment">
<span class="author">じっけんしゃ</span>
{| class="wikitable"
!Name||Sample||Competent Cells||Total||Plate
|-
|いでんし||なんちゃら&micro;L||ごにょごにょ&micro;L||ほにゃらら&micro;L||こーせーぶっしつ
|-
|いかどうよう|| || || ||
|-
| || || || ||
|}
</div>
<!-- ここまでをコピーしてね -->

===Restriction Enzyme Digestion===
<!-- こっから -->
<div class="experiment">
<span class="author">じっけんしゃ</span>
{| class="wikitable"
! ||DNA||restricion enzyme||restriction enzyme||Buffer||BSA||MilliQ||total
|-
|2 cuts||（°°） &micro;L|| &micro;L|| &micro;L|| &micro;L|| &micro;L|| &micro;L|| &micro;L
|-
|NC|| &micro;L|| &micro;L|| &micro;L|| &micro;L|| &micro;L|| &micro;L|| &micro;L
|}
</div>
<!-- ここまでをコピーしてね -->

===Electrophoresis===
<div class="experiment">
<span class="author">Tatsui</span>
{| class="wikitable"
!Lane||Sample||Enzyme1||Enzyme2
|-
|1||100bp ladder
|-
|2||RBS-luxI-DT
|-
|3||Pbad/araC
|-
|4||NC
|-
|5||100bp ladder|}
[[File:igku_xxxxxx.xxx]]<br>
</div>

===Ligation===
 
<!-- こっから -->
<div class="experiment">
<span class="author">じっけんしゃ</span>
{| class="wikitable"
!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2
|-
|experiment||name||xx &micro;L||name||xx &micro;L||xx &micro;L
|-
|NC||name||xx &micro;L||name||xx &micro;L||xx &micro;L
|}
</div>
<!-- ここまでをコピーしてね -->

===Gel Extraction===
<!-- こっから -->
<div class="experiment">
<span class="author">じっけんしゃ</span>
{| class="wikitable"
!Lane||DNA||Enzyme
|-
|（＞ω・）b || ||
|-
| || ||
|}
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxafterxx.xxx]]<br>
{| class="wikitable"
!Name||concentration[&micro;g/mL]||260/280||260/230
|-
|（´ω｀）|| || ||
|-
|（ΦωΦ^）|| || ||
|}
</div>
<!-- ここまでをコピーしてね -->

===Colony PCR===
<!-- ここから -->
<div class="experiment">
<span class="author">No name</span>
{| class="wikitable"
!Sample||base pair
|-
|(｀・ω・´)||
|}
{| class="wikitable"
!PreDenature||Denature||Annealing||Extension||cycle
|-
|　&deg;C||　&deg;C||　&deg;C||　&deg;C||--
|-
|（◎Д◎）　||　||　||　||
|}
</div>
<!-- ここまでをコピーしてね -->
===PCR===
<!-- ここから -->
<div class="experiment">
<span class="author">No name</span>
{| class="wikitable"
!genome DNA||KOD plus||10x buffer||dNTP||MgSO4||SasA_fwd primer||SasA_rev primer||MilliQ||total
|-
| ||0.5||2.5||2.5||1.5||0.75||0.75|| ||25
|}
{| class="wikitable"
!PreDenature||Denature||Annealing||Extension||cycle
|-
|　&deg;C||　&deg;C||　&deg;C||　&deg;C||--
|-
|（◎Д◎）　||　||　||　||
|}
</div>
<!-- ここまでをコピーしてね -->

===Liquid Culture===
<!-- ここから -->
<div class="experiment">
<span class="author">No name</span>
{| class="wikitable"
!Sample||medium
|-
|(・ω・) || 
|-
|（・Ｘ・） ||　
|}
</div>
<!-- ここまでをコピーしてね -->

===Miniprep===
<div class="experiment">
<span class="author">No name</span>
{|class="wikitable" 
!DNA||concentration[&micro;g/mL]||260/280||260/230 
|-
|(・▽・)||　||　||
|}
</div>
<!-- ここまでをコピーしてね -->
===Ethanol Precipitation===
<div class="experiment">
<span class="author">No name</span>
{|class="wikitable" 
!DNA||concentration[&micro;g/mL]||260/280||260/230 
|-
| || || ||
|}
</div>
<!-- ここまでをコピーしてね -->

===Master Plate===
<!-- ここから -->
<div class="experiment">
<span class="author">No name</span>
{| class="wikitable"
!Number||Use LB plate (+抗生物質)
|-
| ʅ（´◔౪◔）ʃ||
|} 
</div>
<!-- ここまでをコピーしてね -->

===Medium Plate===
<div class="experiment">
<span class="author">Nakamoto</span>
{| class="wikitable"
!volume||xx ml
|-
| || 　
|}
</div>
{{Kyoto/Notebook/Aug_8}}
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{{Kyoto/Notebook/Sep_26}}
{{Kyoto/Notebook/Sep_27}}
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