Template:Kyoto/Notebook/Sep 17

(Difference between revisions)

Revision as of 01:59, 24 September 2013

Tatsui and Hirano

Sample	medium
Spi-DT	LB medium(+CP)
Pcon-Spi-DT,Pcon-pT181-anti-spi-DT	LB medium(+Amp)

Hirano

Pcon-tet ap-DT(VF)	BigDye	5x buffer	primer(VF)	MilliQ	total
1.3	0.5	1.75	0.5	0.45	10.5

PreDenature	Denature	Annealing	Extension	cycle
96°C	96°C	50°C	60°C	--
2m	10s	5s	4m	30cycles

Pcon-tet ap-DT(VR)	BigDye	5x buffer	primer(VR)	MilliQ	total
1.3	0.5	1.75	0.5	0.45	10.5

PreDenature	Denature	Annealing	Extension	cycle
96°C	96°C	50°C	60°C	--
2m	10s	5s	4m	30cycles

@@ Line 14: / Line 14: @@
 <!-- ここから -->
 <div class="experiment">
-<span class="author">No name</span>
+<span class="author">Hirano</span>
 {| class="wikitable"
-!genome DNA||KOD plus||10x buffer||dNTP||MgSO4||SasA_fwd primer||SasA_rev primer||MilliQ||total
+!Pcon-tet ap-DT(VF)||BigDye||5x buffer||primer(VF)||MilliQ||total
 |-
-| ||0.5||2.5||2.5||1.5||0.75||0.75|| ||25
+|1.3||0.5||1.75||0.5||0.45||10.5
 |}
 {| class="wikitable"
 !PreDenature||Denature||Annealing||Extension||cycle
 |-
-|　&deg;C||　&deg;C||　&deg;C||　&deg;C||--
+|96&deg;C||96&deg;C||50&deg;C||60&deg;C||--
 |-
-|（◎Д◎）　||　||　||　||
+|2m||10s||5s||4m||30cycles
+|}
+</div>
+<!-- ここまでをコピーしてね --><!-- ここから -->
+<div class="experiment">
+{| class="wikitable"
+!Pcon-tet ap-DT(VR)||BigDye||5x buffer||primer(VR)||MilliQ||total
+|-
+|1.3||0.5||1.75||0.5||0.45||10.5
+|}
+{| class="wikitable"
+!PreDenature||Denature||Annealing||Extension||cycle
+|-
+|96&deg;C||96&deg;C||50&deg;C||60&deg;C||--
+|-
+|2m||10s||5s||4m||30cycles
 |}
 </div>
 <!-- ここまでをコピーしてね -->