Template:Kyoto/Notebook/Sep 17

From 2013.igem.org

(Difference between revisions)

Revision as of 02:39, 24 September 2013

Sep 17

Liquid Culture

Tatsui and Hirano

Sample	medium
Spi-DT	LB medium(+CP)
Pcon-Spi-DT,Pcon-pT181-anti-spi-DT	LB medium(+Amp)

PCR

Hirano

Pcon-tet ap-DT(VF)	BigDye	5x buffer	primer(VF)	MilliQ	total
1.3	0.5	1.75	0.5	0.45	10.5

PreDenature	Denature	Annealing	Extension	cycle
96°C	96°C	50°C	60°C	--
2m	10s	5s	4m	30cycles

Pcon-tet ap-DT(VR)	BigDye	5x buffer	primer(VR)	MilliQ	total
1.3	0.5	1.75	0.5	0.45	10.5

PreDenature	Denature	Annealing	Extension	cycle
96°C	96°C	50°C	60°C	--
2m	10s	5s	4m	30cycles

Ligation

No Name

state	Vector		Inserter		Ligation High ver.2
8/28	name	xx µg/mL	name	xx µg/mL	xx µg/mL
NC	name	xx µg/mL	name	xx µg/mL	xx µg/mL

Gel Extraction

No Name

Lane	DNA	Enzyme
1	1kbpladder	--
2	Pt181 atte -(2)	E+S
3
4

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	concentration[µg/mL]	260/280	260/230
PT181-atte E+S	9.2	1.15	0.12

Lane	DNA	Enzyme
1	1kbpladder	--
2	pSB1C3	E+S
3
4

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	concentration[µg/mL]	260/280	260/230
pSB1C3	10.0	1.84	0.40

Lane	DNA	Enzyme
1	1kbpladder	--
2	pSB4K5	X+P
3
4

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	concentration[µg/mL]	260/280	260/230
pSB4K5	7.6	1.81	0.32

Lane	DNA	Enzyme
1	1kbpladder	--
2	Pcon-RBS-tetR-DT	X+P
3
4

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	concentration[µg/mL]	260/280	260/230
Pcon-RBS-tetR-DT	8.0	2.40	1.14

Lane	DNA	Enzyme
1	1kbpladder	--
2	pSB1C3	E+P
3
4

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	concentration[µg/mL]	260/280	260/230
pSB1C3	9.1	2.17	0.35

PCR

Kinose

pC KaiABC(100pg)	Prime STAR(2x)	Primer(KaiA-Bsai MuT-Fwd)	Primer(KaiA-Bsai MuT-Rev)	MilliQ	total
1.0	25.0	1.0	1.0	22.0	50.0

PreDenature	Denature	Annealing	Extension	cycle
96°C	98°C	65°C	72°C	--
5m	10s	15s	40s	30cycles

pC KaiABC(150pg)	Prime STAR(2x)	Primer(KaiA-Bsai MuT-Fwd)	Primer(KaiA-Bsai MuT-Rev)	MilliQ	total
1.5	25.0	1.0	1.0	21.5	50.0

PreDenature	Denature	Annealing	Extension	cycle
96°C	98°C	65°C	72°C	--
5m	10s	15s	40s	30cycles

</div>

pC KaiABC(200pg)	Prime STAR(2x)	Primer(KaiA-Bsai MuT-Fwd)	Primer(KaiA-Bsai MuT-Rev)	MilliQ	total
2	25.0	1.0	1.0	21.0	50.0

PreDenature	Denature	Annealing	Extension	cycle
96°C	98°C	65°C	72°C	--
5m	10s	15s	40s	30cycles

@@ Line 163: / Line 163: @@
 <!-- ここから -->
 <div class="experiment">
-<span class="author">No name</span>
+<span class="author">Kinose</span>
 {| class="wikitable"
-!genome DNA||KOD plus||10x buffer||dNTP||MgSO4||SasA_fwd primer||SasA_rev primer||MilliQ||total
+!pC KaiABC(100pg)||Prime STAR(2x)||Primer(KaiA-Bsai MuT-Fwd)||Primer(KaiA-Bsai MuT-Rev)||MilliQ||total
 |-
-| ||0.5||2.5||2.5||1.5||0.75||0.75|| ||25
+|1.0||25.0||1.0||1.0||22.0||50.0
 |}
 {| class="wikitable"
 !PreDenature||Denature||Annealing||Extension||cycle
 |-
-|　&deg;C||　&deg;C||　&deg;C||　&deg;C||--
+|96&deg;C||98&deg;C||65&deg;C||72&deg;C||--
 |-
-|（◎Д◎）　||　||　||　||
+|5m||10s||15s||40s||30cycles
+|}
+</div>
+<div class="experiment">
+{| class="wikitable"
+!pC KaiABC(150pg)||Prime STAR(2x)||Primer(KaiA-Bsai MuT-Fwd)||Primer(KaiA-Bsai MuT-Rev)||MilliQ||total
+|-
+|1.5||25.0||1.0||1.0||21.5||50.0
+|}
+{| class="wikitable"
+!PreDenature||Denature||Annealing||Extension||cycle
+|-
+|96&deg;C||98&deg;C||65&deg;C||72&deg;C||--
+|-
+|5m||10s||15s||40s||30cycles
+|}
+</div>
+<!-- ここまでをコピーしてね -->
+</div>
+<div class="experiment">
+{| class="wikitable"
+!pC KaiABC(200pg)||Prime STAR(2x)||Primer(KaiA-Bsai MuT-Fwd)||Primer(KaiA-Bsai MuT-Rev)||MilliQ||total
+|-
+|2||25.0||1.0||1.0||21.0||50.0
+|}
+{| class="wikitable"
+!PreDenature||Denature||Annealing||Extension||cycle
+|-
+|96&deg;C||98&deg;C||65&deg;C||72&deg;C||--
+|-
+|5m||10s||15s||40s||30cycles
 |}
 </div>
 <!-- ここまでをコピーしてね -->

Template:Kyoto/Notebook/Sep 17

From 2013.igem.org

Revision as of 02:39, 24 September 2013

Contents

Sep 17

Liquid Culture

PCR

Ligation

Gel Extraction

PCR