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Friday, 05 Jul 2013
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(Created page with "=='''7/05/13 Primer Design for kerA biobrick'''== *Designed primers for directed mutagenesis of our kerA biobrick using [http://www.bioinformatics.org/primerx/ PrimerX]: #Primer...")
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(Created page with "=='''7/05/13 Primer Design for kerA biobrick'''== *Designed primers for directed mutagenesis of our kerA biobrick using [http://www.bioinformatics.org/primerx/ PrimerX]: #Primer...")
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Revision as of 07:50, 26 September 2013
7/05/13 Primer Design for kerA biobrick
- Designed primers for directed mutagenesis of our kerA biobrick using [http://www.bioinformatics.org/primerx/ PrimerX]:
- Primer pair 1 (EcoRI mutation, aat --> aac, asparagine)
- a. Forward: 5' GGTTAAAGTACTGAACTCAAGCGGAAGCGG 3'
- b. Reverse: 5' CCGCTTCCGCTTGAGTTCAGTACTTTAACC 3'
- Primer pair 2 (Pst1 mutation, gca-->gcg, alanine)
- a. Forward: 5' GTTGTCGTTGTAGCTGCGGCAGGGAACAGCGGATC 3'
- b. Reverse: 5' GATCCGCTGTTCCCTGCCGCAGCTACAACGACAAC 3'
- Used APE for checking codons and mutating restriction sites
