Team:Groningen/25 June 2013

From 2013.igem.org

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A couple of media are prepared:
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'''Mirjam, Chaline and Inne'''
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<br/>A couple of media are prepared:
<br/>LB medium with agar 1 liter
<br/>LB medium with agar 1 liter
<br/>LB medium divided into 80 ml portions
<br/>LB medium divided into 80 ml portions
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Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 degrees Celsius stove.  
Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 degrees Celsius stove.  
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'''Mirjam and Claudio'''
''B. subtilis'' strain 168 is inoculated O/N at 37 degrees Celsius and every buffer necessary for the extraction of genomic DNA is arranged.
''B. subtilis'' strain 168 is inoculated O/N at 37 degrees Celsius and every buffer necessary for the extraction of genomic DNA is arranged.

Revision as of 11:11, 28 June 2013

Mirjam, Chaline and Inne
A couple of media are prepared:
LB medium with agar 1 liter
LB medium divided into 80 ml portions
70% ethanol
2 liter TBE buffer

Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 degrees Celsius stove.

Mirjam and Claudio B. subtilis strain 168 is inoculated O/N at 37 degrees Celsius and every buffer necessary for the extraction of genomic DNA is arranged.