Team:Heidelberg/Templates/Del week15 L
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(Created page with "==07-08-2013== ===Results Sequencing=== Send the following samples (amplified fragments of ''' ''D. acidovorans'' DSM-39 ''' and backbone pSB4K5 from the partsregistry) to GATC...")
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(Created page with "==07-08-2013== ===Results Sequencing=== Send the following samples (amplified fragments of ''' ''D. acidovorans'' DSM-39 ''' and backbone pSB4K5 from the partsregistry) to GATC...")
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Revision as of 01:10, 30 September 2013
Contents |
07-08-2013
Results Sequencing
Send the following samples (amplified fragments of D. acidovorans DSM-39 and backbone pSB4K5 from the partsregistry) to GATC for sequencing:
- DelL (FS_14 to FS_15) with Primer FS_14
- DelL (FS_14 to FS_15) with Primer FS_15
L-FS 14.txt
Sequencing of DelL(FS_14 to FS_15) with Primer FS_14 |
L-FS 15.txt
Sequencing of DelL(FS_14 to FS_15) with Primer FS_15 |
Amplification of DelL (FS_14 to FS_15; 1.4 kb)
- Reaction
what | µl |
---|---|
D. acidovorans SPH-1 | 1 |
FS_14: (1/10) | 2.5 |
FS_15: (1/10) | 2.5 |
Phusion Master Mix | 25 |
DMSO | 2.5 |
dd H2O | 16.5 |
- Conditions
Biorad MyCycler | ||
---|---|---|
Cycles-PCR | temperature [°C] | Time [s] |
1 | 98 | 10 |
12 | 98 | 1 |
66 ↓ 0.5 | 5 | |
72 | 42 | |
18 | 98 | 1 |
63 | 5 | |
72 | 42 | |
1 | 72 | 12 min |
1 | 4 | inf |
Results:
- Amplification worked very well, we had bright bands on the right height.
- Bands were cut out and DNA purified using QIAquick Gel Extration Kit.
08-08-2013
Concentration measurement of DelL
Fragment | Primer | Date PCR | Concentration |
---|---|---|---|
DelL | FS14-FS15 | 07-08-2013 | 51 ng/µl |
09-08-2013
Amplification of DelL (FS_14 to FS_15; 1.4 kb)
- Reaction
what | µl |
---|---|
D. acidovorans | 1 |
FS_14: (1/10) | 2.5 |
FS_15: (1/10) | 2.5 |
Phusion Master Mix | 25 |
DMSO | 2.5 |
dd H2O | 16.5 |
- Conditions
Biorad MyCycler* | ||
---|---|---|
Cycles-PCR | temperature [°C] | Time [s] |
1 | 98 | 10 |
12 | 98 | 1 |
66 ↓ 0.5 | 5 | |
72 | 42 | |
18 | 98 | 1 |
63 | 5 | |
72 | 42 | |
1 | 72 | 12 min |
1 | 10 | inf |
Results:
- Amplification worked very well, we had bright bands on the right height.
- Bands were cut out and DNA purified using QIAquick Gel Extration Kit.