Team:DTU-Denmark/Notebook

From 2013.igem.org

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__NOTOC__
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==Timeline==
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<div class="overviewPage">
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Our team decided to keep an online notebook for our lab work. Click in the calendar to see the entry for a specific date.
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===January===
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* 2013-01-15 Budget completed
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* 2013-01-29 Human Practices: Workshop for highschool students
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===February===
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* 2013-02-25 Project selected: Conversion of ammonia to nitrous oxide in ''E. coli''
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===March===
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* 2013-03-11 Biobrick workshop hosted for KU and SDU
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* 2013-03-13 Project kickoff meeting
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===April===
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* 2013-04-15 Team contract signed
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* 2013-04-28 Human Practices: Outreach for highschool students
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===May===
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* 2013-05-29 "Hello World" pilot project kicked off
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===June===
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* 2013-06-04 Team competency mapping
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* 2013-06-09 Work on wiki started
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* 2013-06-29 Team BBQ
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===July===
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'''Week 27'''
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* Hello World project is successful.  From a cytoplasmic extraction, we have observed GFP in the periplasm and RFP in the cytoplasm. See a nice picture [[Team:DTU-Denmark/Notebook/6_July_2013|here.]]
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* Toxicity Experiment: experiment complete, initial data analysis complete. See results at: [[Team:DTU-Denmark/ToxicityExperiment|here]]
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'''Week 28'''
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* Began growing ''Nitrosomonas'' culture and made special media for it
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* Designed, ordered and received primers to extract genes from ''Nitrosomonas'' and ''Pseudomonas''
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* Began work on cloning genes out of ''Nitrosomonas europaea'' and ''Pseudomonas aeruginosa''.  All isolations successful except Nir.
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'''Week 29'''
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* Performed first nitrate -> nitrite experiment
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'''Week 30'''
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* Only have colonies for the cytochromes -- other genes do not give cultures
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* Attempting to extract Nir gene in two pieces, since this has not been successful so far
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* Began work on arabinose inducible system
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'''Week 31'''
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* Began work on Synthetic Promoter Library
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* BBQ with KU this Saturday
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===August===
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'''Week 32'''
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* The week no PCR worked -- ran 96 PCRs with varying conditions to debug
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* Began work on modelling
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* Began work on presentation
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'''Week 33'''
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* Began daily meetings to coordinate
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* Arabinose inducible promoter works as of 2013-08-11
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* One Nir fragment has been amplified with USER primers (changed DMSO, Mg, temperature, etc), one to go
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* E coli experiment will be run again this week
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'''Week 34'''
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* Decided on final human practices event
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'''Week 35'''
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* Attended Scandinavian meetup in Odense hosted by SDU team
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* Biobricks in progress
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* Biolector for SPL in progress
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* sequencing for SPL in progress
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* first draft of presentation
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* first draft of poster
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* measure Nir, AMO, HAO transformants
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* modelling of kinetics and design of a reactor: Vanessa
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* Title and abstract finished; Title: Requiem for a Stream
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* Reviewed presentation with Julie, who gave us very good advice on pitching our project
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* measure AMO, HAO transformants complete
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===September===
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'''Week 36'''
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* Have biobricks for AMO and cytochromes (are going to sequence these), the others are coming.
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* Running last biolector experiment today
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* sequencing for SPL is in progress
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* Kristian is working on a page in the parts registry for the parts registry
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* first draft of presentation completed
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* first draft of poster complete
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* modelling of kinetics and design of a reactor: using Continuous Stirred Tank Reactor
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* Began reactor design -- ask Mikael Rordham in 223 for 2 1L chemostats
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'''Week 37'''
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* biobricks -- have 2 that are fine: AMO and cytochromes
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* redo colony pcr with sequencing primers to verify
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* Nir experiment on friday
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* Planning for bioreactor experiment
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* Human Practices: Attended BioPeople event "The Basics and the Best" on IP and patent law
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'''Week 38'''
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* Filmed "Bricks of Knowledge" video on USER cloning for KU team
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* Biobricks mailed
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** tat signal peptide
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** amo
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** cytochromes
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* arabinose promoter biobrick not made -- Kristian and Henrike will make this in the next few days
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* Bioreactor experiment started this morning, will finish at 10pm tonight.
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'''Week 39'''
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* Hackathon intensive to complete wiki 28th of Sept
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* Nir experiment -- Kasia and Ariadne will do this on Thursday
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* Video in progress
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* Reviewed poster v2
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=== October ===
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'''Week 40'''
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* Wiki complete!
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==Lab Book Entries==
==Lab Book Entries==
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{{:Team:DTU-Denmark/Templates/EndPage}}
{{:Team:DTU-Denmark/Templates/EndPage}}

Latest revision as of 11:54, 2 October 2013

Notebook


Our team decided to keep an online notebook for our lab work. Click in the calendar to see the entry for a specific date.

Lab Book Entries

June
MTWTFSS
          [http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/1_June_2013&action=edit 1] [http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/2_June_2013&action=edit 2]
[http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/3_June_2013&action=edit 3] [http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/4_June_2013&action=edit 4] [http://2013.igem.org/Team:DTU-Denmark/Notebook/5_June_2013 5] [http://2013.igem.org/Team:DTU-Denmark/Notebook/6_June_2013 6] [http://2013.igem.org/Team:DTU-Denmark/Notebook/7_June_2013 7] [http://2013.igem.org/Team:DTU-Denmark/Notebook/8_June_2013 8] [http://2013.igem.org/Team:DTU-Denmark/Notebook/9_June_2013 9]
[http://2013.igem.org/Team:DTU-Denmark/Notebook/10_June_2013 10] [http://2013.igem.org/Team:DTU-Denmark/Notebook/11_June_2013 11] [http://2013.igem.org/Team:DTU-Denmark/Notebook/12_June_2013 12] [http://2013.igem.org/Team:DTU-Denmark/Notebook/13_June_2013 13] [http://2013.igem.org/Team:DTU-Denmark/Notebook/14_June_2013 14] [http://2013.igem.org/Team:DTU-Denmark/Notebook/15_June_2013 15] [http://2013.igem.org/Team:DTU-Denmark/Notebook/16_June_2013 16]
[http://2013.igem.org/Team:DTU-Denmark/Notebook/17_June_2013 17] [http://2013.igem.org/Team:DTU-Denmark/Notebook/18_June_2013 18] [http://2013.igem.org/Team:DTU-Denmark/Notebook/19_June_2013 19] [http://2013.igem.org/Team:DTU-Denmark/Notebook/20_June_2013 20] [http://2013.igem.org/Team:DTU-Denmark/Notebook/21_June_2013 21] [http://2013.igem.org/Team:DTU-Denmark/Notebook/22_June_2013 22] [http://2013.igem.org/Team:DTU-Denmark/Notebook/23_June_2013 23]
[http://2013.igem.org/Team:DTU-Denmark/Notebook/24_June_2013 24] [http://2013.igem.org/Team:DTU-Denmark/Notebook/25_June_2013 25] [http://2013.igem.org/Team:DTU-Denmark/Notebook/26_June_2013 26] [http://2013.igem.org/Team:DTU-Denmark/Notebook/27_June_2013 27] [http://2013.igem.org/Team:DTU-Denmark/Notebook/28_June_2013 28] [http://2013.igem.org/Team:DTU-Denmark/Notebook/29_June_2013 29] [http://2013.igem.org/Team:DTU-Denmark/Notebook/30_June_2013 30]
July
MTWTFSS
[http://2013.igem.org/Team:DTU-Denmark/Notebook/1_July_2013 1] [http://2013.igem.org/Team:DTU-Denmark/Notebook/2_July_2013 2] [http://2013.igem.org/Team:DTU-Denmark/Notebook/3_July_2013 3] [http://2013.igem.org/Team:DTU-Denmark/Notebook/4_July_2013 4] [http://2013.igem.org/Team:DTU-Denmark/Notebook/5_July_2013 5] [http://2013.igem.org/Team:DTU-Denmark/Notebook/6_July_2013 6] [http://2013.igem.org/Team:DTU-Denmark/Notebook/7_July_2013 7]
[http://2013.igem.org/Team:DTU-Denmark/Notebook/8_July_2013 8] [http://2013.igem.org/Team:DTU-Denmark/Notebook/9_July_2013 9] [http://2013.igem.org/Team:DTU-Denmark/Notebook/10_July_2013 10] [http://2013.igem.org/Team:DTU-Denmark/Notebook/11_July_2013 11] [http://2013.igem.org/Team:DTU-Denmark/Notebook/12_July_2013 12] [http://2013.igem.org/Team:DTU-Denmark/Notebook/13_July_2013 13] [http://2013.igem.org/Team:DTU-Denmark/Notebook/14_July_2013 14]
[http://2013.igem.org/Team:DTU-Denmark/Notebook/15_July_2013 15] [http://2013.igem.org/Team:DTU-Denmark/Notebook/16_July_2013 16] [http://2013.igem.org/Team:DTU-Denmark/Notebook/17_July_2013 17] [http://2013.igem.org/Team:DTU-Denmark/Notebook/18_July_2013 18] [http://2013.igem.org/Team:DTU-Denmark/Notebook/19_July_2013 19] [http://2013.igem.org/Team:DTU-Denmark/Notebook/20_July_2013 20] [http://2013.igem.org/Team:DTU-Denmark/Notebook/21_July_2013 21]
[http://2013.igem.org/Team:DTU-Denmark/Notebook/22_July_2013 22] [http://2013.igem.org/Team:DTU-Denmark/Notebook/23_July_2013 23] [http://2013.igem.org/Team:DTU-Denmark/Notebook/24_July_2013 24] [http://2013.igem.org/Team:DTU-Denmark/Notebook/25_July_2013 25] [http://2013.igem.org/Team:DTU-Denmark/Notebook/26_July_2013 26] [http://2013.igem.org/Team:DTU-Denmark/Notebook/27_July_2013 27] [http://2013.igem.org/Team:DTU-Denmark/Notebook/28_July_2013 28]
[http://2013.igem.org/Team:DTU-Denmark/Notebook/29_July_2013 29] [http://2013.igem.org/Team:DTU-Denmark/Notebook/30_July_2013 30] [http://2013.igem.org/Team:DTU-Denmark/Notebook/31_July_2013 31]
August
MTWTFSS
      [http://2013.igem.org/Team:DTU-Denmark/Notebook/1_August_2013 1] [http://2013.igem.org/Team:DTU-Denmark/Notebook/2_August_2013 2] [http://2013.igem.org/Team:DTU-Denmark/Notebook/3_August_2013 3] [http://2013.igem.org/Team:DTU-Denmark/Notebook/4_August_2013 4]
[http://2013.igem.org/Team:DTU-Denmark/Notebook/5_August_2013 5] [http://2013.igem.org/Team:DTU-Denmark/Notebook/6_August_2013 6] [http://2013.igem.org/Team:DTU-Denmark/Notebook/7_August_2013 7] [http://2013.igem.org/Team:DTU-Denmark/Notebook/8_August_2013 8] [http://2013.igem.org/Team:DTU-Denmark/Notebook/9_August_2013 9] [http://2013.igem.org/Team:DTU-Denmark/Notebook/10_August_2013 10] [http://2013.igem.org/Team:DTU-Denmark/Notebook/11_August_2013 11]
[http://2013.igem.org/Team:DTU-Denmark/Notebook/12_August_2013 12] [http://2013.igem.org/Team:DTU-Denmark/Notebook/13_August_2013 13] [http://2013.igem.org/Team:DTU-Denmark/Notebook/14_August_2013 14] [http://2013.igem.org/Team:DTU-Denmark/Notebook/15_August_2013 15] [http://2013.igem.org/Team:DTU-Denmark/Notebook/16_August_2013 16] [http://2013.igem.org/Team:DTU-Denmark/Notebook/17_August_2013 17] [http://2013.igem.org/Team:DTU-Denmark/Notebook/18_August_2013 18]
[http://2013.igem.org/Team:DTU-Denmark/Notebook/19_August_2013 19] [http://2013.igem.org/Team:DTU-Denmark/Notebook/20_August_2013 20] [http://2013.igem.org/Team:DTU-Denmark/Notebook/21_August_2013 21] [http://2013.igem.org/Team:DTU-Denmark/Notebook/22_August_2013 22] [http://2013.igem.org/Team:DTU-Denmark/Notebook/23_August_2013 23] [http://2013.igem.org/Team:DTU-Denmark/Notebook/24_August_2013 24] [http://2013.igem.org/Team:DTU-Denmark/Notebook/25_August_2013 25]
[http://2013.igem.org/Team:DTU-Denmark/Notebook/26_August_2013 26] [http://2013.igem.org/Team:DTU-Denmark/Notebook/27_August_2013 27] [http://2013.igem.org/Team:DTU-Denmark/Notebook/28_August_2013 28] [http://2013.igem.org/Team:DTU-Denmark/Notebook/29_August_2013 29] [http://2013.igem.org/Team:DTU-Denmark/Notebook/30_August_2013 30] [http://2013.igem.org/Team:DTU-Denmark/Notebook/31_August_2013 31]
September
MTWTFSS
            [http://2013.igem.org/Team:DTU-Denmark/Notebook/1_September_2013 1]
[http://2013.igem.org/Team:DTU-Denmark/Notebook/2_September_2013 2] [http://2013.igem.org/Team:DTU-Denmark/Notebook/3_September_2013 3] [http://2013.igem.org/Team:DTU-Denmark/Notebook/4_September_2013 4] [http://2013.igem.org/Team:DTU-Denmark/Notebook/5_September_2013 5] [http://2013.igem.org/Team:DTU-Denmark/Notebook/6_September_2013 6] [http://2013.igem.org/Team:DTU-Denmark/Notebook/7_September_2013 7] [http://2013.igem.org/Team:DTU-Denmark/Notebook/8_September_2013 8]
[http://2013.igem.org/Team:DTU-Denmark/Notebook/9_September_2013 9] [http://2013.igem.org/Team:DTU-Denmark/Notebook/10_September_2013 10] [http://2013.igem.org/Team:DTU-Denmark/Notebook/11_September_2013 11] [http://2013.igem.org/Team:DTU-Denmark/Notebook/12_September_2013 12] [http://2013.igem.org/Team:DTU-Denmark/Notebook/13_September_2013 13] [http://2013.igem.org/Team:DTU-Denmark/Notebook/14_September_2013 14] [http://2013.igem.org/Team:DTU-Denmark/Notebook/15_September_2013 15]
[http://2013.igem.org/Team:DTU-Denmark/Notebook/16_September_2013 16] [http://2013.igem.org/Team:DTU-Denmark/Notebook/17_September_2013 17] [http://2013.igem.org/Team:DTU-Denmark/Notebook/18_September_2013 18] [http://2013.igem.org/Team:DTU-Denmark/Notebook/19_September_2013 19] [http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/20_September_2013&action=edit 20] [http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/21_September_2013&action=edit 21] [http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/22_September_2013&action=edit 22]
[http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/23_September_2013&action=edit 23] [http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/24_September_2013&action=edit 24] [http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/25_September_2013&action=edit 25] [http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/26_September_2013&action=edit 26] [http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/27_September_2013&action=edit 27] [http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/28_September_2013&action=edit 28] [http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/29_September_2013&action=edit 29]
[http://2013.igem.org/wiki/index.php?title=Team:DTU-Denmark/Notebook/30_September_2013&action=edit 30]