Template:Team:Bonn:NetworkData

content.text= "<p>At the moment our system is only working in sspB-deficient bacteria as the natural occurrence of sspB in E. coli interferes with our split-sspB system. This problem could be solved by using the core region of sspB and the ssrA tag from the Caulobacter crescentus (Cc) bacteria. That way proteins with the Cc-ssrA tag can be degraded by the ClpXP protease after a light stimulus while the natural protein degradation homeostasis is not influenced. In order to do so, a split version of Cc-sspB would have to be generated and implemented.</p><p>With the Cc versions the kill-switch system could be used as a general lab safety tool for genetically engineered organisms (GEO). Despite of current safety measures, it can never be guaranteed that GEOs escape the laboratories. The kill switch would in that case ensure that the escaped organisms die and do not pose a threat to people and nature.</p><p>The next step would be to implement our degradation system in eukaryotes such as yeast. The ClpXP protease and the light-regulated sspB can be expressed in yeast. Proteins that were tagged with ssrA would then be degraded at a light signal. That way the kill-switch is also possible in eukaryotes.</p> ";