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Team:Heidelberg/Templates/MM week14p
From 2013.igem.org
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Nils.kurzawa (Talk | contribs) m (Created page with " == 2013-07-29 == 1 µl of pLF03 miniPrep from 2013-06-04 * make miniPrep -> very low yield (1-2 ng/µl) * load 1µl of 97ng/µl pLF0...") |
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== 2013-07-29 == | == 2013-07-29 == | ||
| - | [[File: | + | [[File:Heidelberg_PLF03-20130729.png|100px|thumb|1 µl of pLF03 miniPrep from 2013-06-04]] |
* make miniPrep -> very low yield (1-2 ng/µl) | * make miniPrep -> very low yield (1-2 ng/µl) | ||
* load 1µl of 97ng/µl pLF03 miniPrep from 2013-06-04 on gel -> ca. 20 ng/µl | * load 1µl of 97ng/µl pLF03 miniPrep from 2013-06-04 on gel -> ca. 20 ng/µl | ||
| Line 7: | Line 7: | ||
== 2013-07-30 == | == 2013-07-30 == | ||
| - | [[File: | + | [[File:Heidelberg_PLF03-20130730.png|100px|thumb|1 µl of pLF03 midiPrep from 2013-07-30]] |
* make midiPrep -> 1352 ng/µl in 66.5 µl | * make midiPrep -> 1352 ng/µl in 66.5 µl | ||
* medium was contaminated (also check with Fanny) -> low yields of previous miniPreps | * medium was contaminated (also check with Fanny) -> low yields of previous miniPreps | ||
== 2013-07-31 == | == 2013-07-31 == | ||
| - | * send 20 µl of 1:15 dilution of midiPrep to sequencing with primers IK07, IK36, IK37: [[File: | + | * send 20 µl of 1:15 dilution of midiPrep to sequencing with primers IK07, IK36, IK37: [[File:Heidelberg_PLF03 seq 2013-07-31.zip]] |
| - | * perform multiple sequence alignment against assumed pLF03 sequence: [[:File: | + | * perform multiple sequence alignment against assumed pLF03 sequence: [[:File:Heidelberg_PLF03-2013-07-31_IK07.clustal.txt|IK07]], [[:File:Heidelberg_PLF03-2013-07-31_IK36.clustal.txt|IK36]], [[:File:Heidelberg_PLF03-2013-07-31_IK37.clustal.txt|IK37]] |
* deletion at the end of accA2 -> primer can not bind | * deletion at the end of accA2 -> primer can not bind | ||
Latest revision as of 02:34, 5 October 2013
2013-07-29
- make miniPrep -> very low yield (1-2 ng/µl)
- load 1µl of 97ng/µl pLF03 miniPrep from 2013-06-04 on gel -> ca. 20 ng/µl
- inoculate 50 ml LB+Amp with TOP10-pLF03, grow at 37°C
2013-07-30
- make midiPrep -> 1352 ng/µl in 66.5 µl
- medium was contaminated (also check with Fanny) -> low yields of previous miniPreps
2013-07-31
- send 20 µl of 1:15 dilution of midiPrep to sequencing with primers IK07, IK36, IK37: File:Heidelberg PLF03 seq 2013-07-31.zip
- perform multiple sequence alignment against assumed pLF03 sequence: IK07, IK36, IK37
- deletion at the end of accA2 -> primer can not bind
