Team:Warsaw/Glossary

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{{:Team:Warsaw/Templates/StandardPageBegin|Project description}}
{{:Team:Warsaw/Templates/StandardPageBegin|Project description}}
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__TOC__
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==A==
==A==
* '''acrylamide''' – our subject of study
* '''acrylamide''' – our subject of study
==B==
==B==
* '''BiFC''' – Bimolecular Fluorescent Complementation
* '''BiFC''' – Bimolecular Fluorescent Complementation
-
C cloning – on pSB1C3 of course!
+
==C==
-
D
+
* '''cloning''' – on pSB1C3 of course!
-
E ethanol – lots of ethanol in cell lab!
+
* '''calm resignation''' – general mood two days prior to wiki freeze (see 'panic attack')
-
F FluoSafe – our project
+
*m'''Cherry''' - red fluorescent protein. We managed to succesfully clone C- and N-terminal BiFC fragments into pSB1C3 and deliver them to the Registry
-
G Generacja Przyszłości (eng. Generation of the Future) – our grant
+
 
-
H HeLa and HEK – cells we worked with
+
==D==
-
I
+
* '''despair''' - general mood week prior to wiki freeze (see 'calm resignation')
-
J
+
 
-
K
+
==E==
-
L
+
* '''ethanol''' – lots of ethanol in cell lab (take a deep breath ladies and gentelmen - unless one of you is the designated driver)
-
M
+
 
-
N
+
==F==
-
O
+
* '''FedEx''' - delivery services, which tried to take our precious samples, results of our hard work
-
P PCR – polymerase chain reaction
+
* '''FluoSafe''' – our project
-
Q
+
 
-
R
+
==G==
-
S superfolder proteins
+
* Airport de '''Gaulle'a''' - the place, where our parts got held up a few hours too long
-
T
+
* '''Generacja Przyszłości (eng. ''Generation of the Future'')''' – our grant
-
U
+
 
-
V
+
==H==
-
W wirówka (eng. centrifuge) – that we unintentionally! broke
+
* '''HeLa and HEK''' – cells we worked with
-
X
+
* '''hemoglobin''' - core of our sensor
-
Y
+
 
-
Z
+
==I==
 +
* '''irritation''' - when this **** C-m6 and C-m12 cannot clone in pSB1C3 plasmid!
 +
 
 +
==J==
 +
* '''Jamboree''' - we're waiting for it
 +
 
 +
==K==
 +
* '''kill-switch''' - our safety system which wasn't synthetized on time (see: 'lysis device' and 'MinC')
 +
 
 +
==L==
 +
* '''Lyon''' - we can send you postcard from jamboree
 +
* '''lysis device''' - another component of our kill-switch (see: 'MinC')
 +
 
 +
==M==
 +
* '''MinC''' - core of our kill-switch. Inhibits bacterial division.
 +
 
 +
==N==
 +
*'''N-terminal valine''' - acrylamide forms adducts on the N-terminus of hemoglobin alpha and beta chains. Adducts interfere with tetramer formation. We attempted to take advantage of this property while designing the hemoglobin-based acrylamide sensor.
 +
 
 +
==O==
 +
*m'''Orange''' - we nearly managed to produce BiFC fragments form this fluorescent protein
 +
 
 +
==P==
 +
* '''PCR''' – polymerase chain reaction
 +
* '''panic attack''' – begins approximately 12 h prior to wiki freeze
 +
 
 +
==Q==
 +
*'''questionable''' - large portion of our clones and PCR products
 +
 
 +
==R==
 +
* '''roGFP''' - redox-sensitive GFP
 +
 
 +
==S==
 +
* '''superfolder proteins''' - improved derivatives of ''A. victoria''-based fluorescent proteins (green, yellow, blue and cyan). This part of our project actually succeded. They're brighter, fun to work with and considerably more stable (as a poet once said: 'harder, better, faster, stronger' - and our work with them still isn't over)
 +
 
 +
==T==
 +
* '''Toolbox (BiFC's)''' - one of our goals
 +
 
 +
==U==
 +
* '''ultraviolet''' - and GFP glows
 +
* '''The Unknown Sample''' - not all abbreviations are as obvious now as there were a month ago...
 +
 
 +
==V==
 +
* '''vacation''' - in lab all the time!
 +
 
 +
==W==
 +
* '''wirówka (eng. ''centrifuge'')''' – that we unintentionally broke!
 +
* '''wiki freeze''' – the ultimate deadline (see: "despair', 'calm resignation' and 'panic attack')
 +
 
 +
==X==
 +
*'''X-Files''' - paranormal no longer seems impressive, once you've seen some of our results...
 +
 
 +
==Y==
 +
* '''yellow fluorescent proteins (sfYFP and SYFP2)''' - and their glowing rivalry!
 +
 
 +
==Z==
 +
*'''Zombie walk''' - team departs the lab following wiki freeze
{{:Team:Warsaw/Templates/StandardPageEnd}}
{{:Team:Warsaw/Templates/StandardPageEnd}}

Latest revision as of 03:46, 5 October 2013

Project description

Contents

A

  • acrylamide – our subject of study

B

  • BiFC – Bimolecular Fluorescent Complementation

C

  • cloning – on pSB1C3 of course!
  • calm resignation – general mood two days prior to wiki freeze (see 'panic attack')
  • mCherry - red fluorescent protein. We managed to succesfully clone C- and N-terminal BiFC fragments into pSB1C3 and deliver them to the Registry

D

  • despair - general mood week prior to wiki freeze (see 'calm resignation')

E

  • ethanol – lots of ethanol in cell lab (take a deep breath ladies and gentelmen - unless one of you is the designated driver)

F

  • FedEx - delivery services, which tried to take our precious samples, results of our hard work
  • FluoSafe – our project

G

  • Airport de Gaulle'a - the place, where our parts got held up a few hours too long
  • Generacja Przyszłości (eng. Generation of the Future) – our grant

H

  • HeLa and HEK – cells we worked with
  • hemoglobin - core of our sensor

I

  • irritation - when this **** C-m6 and C-m12 cannot clone in pSB1C3 plasmid!

J

  • Jamboree - we're waiting for it

K

  • kill-switch - our safety system which wasn't synthetized on time (see: 'lysis device' and 'MinC')

L

  • Lyon - we can send you postcard from jamboree
  • lysis device - another component of our kill-switch (see: 'MinC')

M

  • MinC - core of our kill-switch. Inhibits bacterial division.

N

  • N-terminal valine - acrylamide forms adducts on the N-terminus of hemoglobin alpha and beta chains. Adducts interfere with tetramer formation. We attempted to take advantage of this property while designing the hemoglobin-based acrylamide sensor.

O

  • mOrange - we nearly managed to produce BiFC fragments form this fluorescent protein

P

  • PCR – polymerase chain reaction
  • panic attack – begins approximately 12 h prior to wiki freeze

Q

  • questionable - large portion of our clones and PCR products

R

  • roGFP - redox-sensitive GFP

S

  • superfolder proteins - improved derivatives of A. victoria-based fluorescent proteins (green, yellow, blue and cyan). This part of our project actually succeded. They're brighter, fun to work with and considerably more stable (as a poet once said: 'harder, better, faster, stronger' - and our work with them still isn't over)

T

  • Toolbox (BiFC's) - one of our goals

U

  • ultraviolet - and GFP glows
  • The Unknown Sample - not all abbreviations are as obvious now as there were a month ago...

V

  • vacation - in lab all the time!

W

  • wirówka (eng. centrifuge) – that we unintentionally broke!
  • wiki freeze – the ultimate deadline (see: "despair', 'calm resignation' and 'panic attack')

X

  • X-Files - paranormal no longer seems impressive, once you've seen some of our results...

Y

  • yellow fluorescent proteins (sfYFP and SYFP2) - and their glowing rivalry!

Z

  • Zombie walk - team departs the lab following wiki freeze